Characteristics of lymphocyte subsets in HIV-infected, long-term nonprogressor, and healthy Asian children through 12 years of age
Characteristics of lymphocyte subsets in HIV-infected, long-term nonprogressor, and healthy Asian children through 12 years of age
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DOI：
10.1016/j.jaci.2010.09.038
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发表时间：
2010-12-01
影响因子：
14.2
通讯作者：
Shearer, William T.
中科院分区：
文献类型：
--
作者：
Ananworanich, Jintanat;Apornpong, Tanakorn;Shearer, William T.
背景资料：与健康对照组相比，HIV阳性儿童的免疫特征数据有限，而亚洲儿童则没有这样的数据。目的：与年龄匹配的健康对照组相比，对HIV阳性亚洲儿童（包括长期非进展者（LTNP））进行免疫表型分析。方法：我们用流式细胞仪分析了222例未经治疗的患者的13个淋巴细胞和单核细胞亚群，HIV阳性儿童，CD 4（+）T细胞15%至24%，无艾滋病相关疾病和142名健康儿童（对照组）。对各年龄组的数据进行了比较。将LTNP（n = 50）（定义为CD 4（+）T细胞计数>= 350个/mm 3的8岁以上儿童）的资料与年龄匹配的非LTNP（n = 17）和对照组（n = 53）的资料进行比较。与对照组相比，HIV阳性儿童的数值较低（每mm（3）细胞计数和分布百分比），细胞毒性T细胞的值较高，初始T-H和细胞毒性T细胞群体减少，B细胞和自然杀伤（NK）细胞。HIV阳性儿童的活化T-H和细胞毒性T细胞值较高。与非LTNP相比，LTNP具有更高的T-H和细胞毒性T细胞、初始和记忆T细胞亚群以及B和NK细胞值。令人惊讶的是，LTNP中活化的T-H和细胞毒性T细胞的计数也较高。结论：未经治疗，HIV感染的亚洲儿童的免疫概况，不同于对照组，其特点是低值的T-H细胞，幼稚T细胞，B细胞，NK细胞，但高值的细胞毒性，活化的T-H细胞和细胞毒性T细胞。在LTNP中观察到的活化T细胞的较高值需要在纵向研究中确认。（J Allergy Clin Immunol 2010;126：1294-301.）
Background: There are limited data on the immune profiles of HIV-positive children compared with healthy controls, and no such data for Asian children.Objectives: To immunophenotype HIV-positive Asian children, including long-term nonprogressors (LTNPs), compared with age-matched healthy controls.Methods: We used flow cytometry to analyze 13 lymphocyte and monocyte subsets from 222 untreated, HIV-positive children with 15% to 24% CD4(+) T cells and no AIDS-related illnesses and 142 healthy children (controls). Data were compared among age categories. Profiles from LTNPs (n = 50), defined as children >= 8 years old with CD4(+) T-cell counts >= 350 cells/mm(3), were compared with data from age-matched non-LTNPs (n = 17) and controls (n = 53).Results: Compared with controls, HIV-positive children had lower values (cell count per mm(3) and percent distribution) for T-H cells and higher values for cytotoxic T cells, with reductions in populations of naive T-H and cytotoxic T cells, B cells, and natural killer (NK) cells. HIV-positive children had high values for activated T-H and cytotoxic T cells. Compared with non-LTNPs, LTNPs had higher values of T-H and cytotoxic T cells, naive and memory T-cell subsets, and B and NK cells. Surprisingly, counts of activated T-H and cytotoxic T cells were also higher among LTNPs. LNTPs were more frequently male.Conclusion: Untreated, HIV-infected Asian children have immune profiles that differ from those of controls, characterized by low values for T-H cells, naive T cells, B cells, and NK cells but high values for cytotoxic, activated T-H, and cytotoxic T cells. The higher values for activated T cells observed in LTNPs require confirmation in longitudinal studies. (J Allergy Clin Immunol 2010;126:1294-301.)