PREDICTION OF PROTEIN ANTIGENIC DETERMINANTS FROM AMINO-ACID-SEQUENCES
PREDICTION OF PROTEIN ANTIGENIC DETERMINANTS FROM AMINO-ACID-SEQUENCES
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DOI：
10.1073/pnas.78.6.3824
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
WOODS, KR
中科院分区：
文献类型：
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作者：
HOPP, TP;WOODS, KR
提出了一种通过分析氨基酸序列找到最大局部亲水性点来定位蛋白质抗原决定簇的方法。这是通过为每个氨基酸指定一个数值（亲水性值），然后沿着肽链重复对这些值进行平均来实现的。最高局部平均亲水性点总是位于抗原决定簇中或紧邻抗原决定簇。预测成功率取决于平均组长度，六肽平均值可产生最佳结果。该方法使用 12 种蛋白质进行了广泛的免疫化学分析，随后用于预测以下蛋白质的抗原决定簇：乙型肝炎表面抗原、流感血凝素、鸡瘟病毒血凝素、人组织相容性抗原 HLA-B7、人干扰素、大肠杆菌和霍乱 肠毒素、豚草过敏原 Ra3 和 Ra5 以及链球菌 M 蛋白。采用化学方法合成乙型肝炎表面抗原序列，并经放射免疫分析显示具有抗原活性。
A method is presented for locating protein antigenic determinants by analyzing amino acid sequences to find the point of greatest local hydrophilicity. This is accomplished by asigning each amino acid a numerical value (hydrophilicity value) and then repetitively averaging these values along the peptide chain. The point of highest local average hydrophilicity is invariably located in, or immediately adjacent to, an antigenic determinant. The prediction success rate depended on averaging group length, with hexapeptide averages yielding optimal results. The method was developed using 12 proteins for which extensive immunochemical analysis has been carried out and subsequently was used to predict antigenic determinants for the following proteins: hepatitis B surface antigen, influenza hemagglutinins, fowl plague virus hemagglutinin, human histocompatibility antigen HLA-B7, human interferons, Escherichia coli and cholera enterotoxins, ragweed allergens Ra3 and Ra5 and streptococcal M protein. The hepatitis B surface antigen sequence was synthesized by chemical means and was shown to have antigenic activity by radioimmunoassay.