Role of glutathione and hsp 70 in the acquisition of thermotolerance in postimplantation rat embryos.
Role of glutathione and hsp 70 in the acquisition of thermotolerance in postimplantation rat embryos.
复制标题
谷胱甘肽和热休克蛋白 70 在植入后大鼠胚胎获得耐热性中的作用。
DOI：
10.1002/tera.1420430307
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Mirkes,PE
中科院分区：
文献类型：
--
作者：
Harris,C;Juchau,MR;Mirkes,PE
启动研究以确定还原型谷胱甘肽（GSH）可能参与培养的大鼠胚胎对暴露于高温（热休克）的有害影响产生热诱导耐受性的能力的程度。对胚胎对热休克（43°C，30 min）的畸形反应以及对hsp 70基因表达和随后的hsps形成的调节的研究表明，大鼠胚胎获得热耐受性可能受到GSH合成抑制的显著影响。用L-丁硫基-九-S，R-亚砜亚胺（BSO）处理孕体降低了细胞内GSH浓度，并损害了胚胎产生耐热反应的能力，这通过生长和发育指数的改变来评估。在42°C下预暴露30分钟所引起的胚胎耐热性伴随着GSH水平的增加，其水平高于在随后的43°C热暴露之前在37°C下对照胚胎中测量的水平。在温度升高至42°C后不久即可检测到hsp 70 mRNA的表达，并在43°C热休克后1.5小时达到其最高积累水平。BSO处理对hsp 70信息水平或hsp 70的合成几乎没有影响。BSO处理减弱了热耐受性反应，但没有产生hsp 70 RNA或hsp 70合成的减少，这表明单独的hsp 70不足以赋予培养的大鼠胚胎热耐受性。
Studies were initiated to determine the extent to which reduced glutathione (GSH) may be involved in the capacity of cultured rat embryos to develop heat‐induced tolerance to the deleterious effects of exposure to high temperatures (heat shock). Investigations of the modulation of dysmorpho‐genic responses of embryos to heat shock (43°C, 30 min) as well as to the expression of the hsp70 gene and subsequent formation of hsps indicated that the acquisition of thermotolerance by rat embryos could be significantly influenced by the inhibition of GSH synthesis. Treatment of conceptuses with L‐buthio‐nine‐S,R‐sulfoximine (BSO) reduced intracellular GSH concentrations and compromised the capacity of embryos to mount a thermotolerance response as assessed by alterations in indices of growth and development. Embryonic thermotolerance elicited by preexposure to 42°C for 30 min was accompanied by increases in GSH to levels greater than those measured in control embryos at 37°C just prior to the subsequent 43°C heat exposure. Expression of hsp70 mRNA was detectable soon after elevation of the temperature to 42°C and reached its highest level of accumulation 1.5 hr after the 43°C heat shock. BSO treatment had little if any effect on hsp70 message levels or on the synthesis of hsp70. The fact that BSO‐treatment attenuated the thermotolerance response but did not produce a decrease in hsp70 RNA or the synthesis of hsp70 suggests that hsp70 alone is not sufficient to confer thermotolerance upon cultured rat embryos.