Association of HLA class II alleles with hepatitis C virus clearance and persistence in thalassemia patients from Iran
Association of HLA class II alleles with hepatitis C virus clearance and persistence in thalassemia patients from Iran
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DOI：
10.1002/jmv.24211
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发表时间：
2015-09-01
影响因子：
12.7
通讯作者：
Rahimnia, Ramin
中科院分区：
文献类型：
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作者：
Samimi-Rad, Katayoun;Sadeghi, Farzin;Rahimnia, Ramin
目前还没有关于HLA II类等位基因与伊朗患者急性丙型肝炎病毒（HCV）感染后清除或持续存在的关联的公开数据。采用序列特异性引物聚合酶链反应扩增技术（PCR-SSP）检测117例地中海贫血患者（63例慢性感染，54例病毒清除）和120例健康对照者的HLA DRB1、DQA1和DQB1等位基因。HCV清除率患者中HLA-DRB1*0301和DQA1*0501等位基因的存在显著高于慢性感染患者（P=0.03和P=0.0007）。与病毒清除组相比，慢性感染组存在DRB1* 07001、DQA1*0201和DQB1*0602等位基因，差异有统计学意义（P=0.004、P=0.007和P=0.02）。与对照组相比，慢性患者DRB1*0301、DRB1*11、DQA1*0501、DQB1*0301等位基因的表达显著降低（P=0.002、P=0.001、P=0.0001、P=0.0004）。HCV清除率患者DRB1*0301、DQA1*0501、DQB1*0201和DRB1*1101、DQA1*0501、DQB1*0301的单倍型频率显著高于慢性感染患者（P=0.004和P=0.04）。而DRB1*0701、DQA1*0201、DQB1*0201单倍型在慢性感染人群中的发生率高于病毒清除人群（P=0.02）。这些发现表明，特定的HLA等位基因和相关的单倍型可能对伊朗患者的HCV感染结果有影响。在伊朗患者中发现的一些HLA等位基因与其他地方报道的不同，这表明HCV清除或持久性的免疫遗传组成可能因种族而异。中华医学杂志。87:1565-1572,2015。(c) 2015 Wiley期刊公司
There is no published data on association of HLA class II alleles with clearance or persistence after acute hepatitis C virus (HCV) infection in patients from Iran. HLA DRB1, DQA1, and DQB1 alleles were determined using polymerase chain reaction amplification with sequence specific primers (PCR-SSP) on a total of 117 thalassemia patients (63 with chronic infection, and 54 with viral clearance) and 120 healthy controls. HLA-DRB1*0301 and DQA1*0501 alleles were found significantly present in patients with HCV clearance compared to those with chronic infection (P=0.03 and P=0.0007, respectively). By contrast, DRB1*0701, DQA1*0201, and DQB1*0602 alleles occurred significantly in those with chronic infection compared to those with viral clearance (P=0.004, P=0.007, and P=0.02, respectively). As compared to the controls, DRB1*0301, DRB1*11, DQA1*0501, and DQB1*0301 alleles showed a significant decrease in chronic patients (P=0.002, P=0.001, P=0.0001, and P=0.0004, respectively). Furthermore, the haplotype frequencies of DRB1*0301, DQA1*0501, DQB1*0201, and DRB1*1101, DQA1*0501, DQB1*0301 were found significantly higher (P=0.004 and P=0.04, respectively) in patients with HCV clearance than those with chronic infection. By contrast, the haplotype DRB1*0701, DQA1*0201, DQB1*0201 occurred more frequently (P=0.02) in those with chronic infection compared with those with viral clearance. These findings suggest that particular HLA alleles and related haplotypes may have an influence on the outcome of HCV infection among the Iranian patients. Some of the HLA alleles found in the Iranian patients are different from those reported elsewhere, suggesting that the immunogenetic makeup for HCV clearance or persistence may vary based on the ethnicity. J. Med. Virol. 87:1565-1572, 2015. (c) 2015 Wiley Periodicals, Inc.