Proliferative changes in the epithelium of the human lithiasic gallabladder.
Proliferative changes in the epithelium of the human lithiasic gallabladder.
复制标题
人类胆囊结石上皮的增殖性变化。
DOI：
10.1093/jnci/60.2.283
复制
发表时间：
1978
期刊：
影响因子：
--
通讯作者：
G. Williams
中科院分区：
文献类型：
--
作者：
P. Putz;G. Williams
用[~ 3 H]胸腺嘧啶核苷对9例正常人胆囊和9例结石胆囊的手术标本进行体外孵育。应用放射自显影技术观察粘膜上皮细胞DNA合成情况。标记细胞的百分比（标记指数）和有丝分裂像（有丝分裂指数）的估计。在正常胆囊中，标记指数低，核分裂象异常，上皮中的标记细胞与基底膜相对。在结石胆囊中，标记指数高23倍（P <0.01），可见一定数量的有丝分裂像;[3 H]胸苷的摄取不限于基底上皮细胞，发生在粘膜的任何水平。我们的数据表明，增殖活性增加的人胆囊结石的粘膜。在一定程度上，对正常胆囊基底膜的标记细胞代表上皮祖细胞，我们的研究结果也表明，在锂胆囊粘膜标记细胞的空间分布的修改。细胞动力学中这种伴随的定量和定性变化可能与消化道中已知的癌前状态平行。
Surgical specimens from 9 normal human gallbladders and from 9 lithiasic gallbladders were incubated in vitro with [3H]thymidine. Autoradiography was used to observe the cells in DNA synthesis in the mucosal epithelium. The percentages of labeled cells (labeling index) and of mitotic figures (mitotic index) were estimated. In the normal gallbladder, the labeling index was low and mitotic figures were exceptional; the labeled cells in the epithelium were observed against the basal membrane. In the lithiasic gallbladder, the labeling index was 23 times higher (P less than 0.01) and a definite number of mitotic figures was seen; the uptake of [3H]thymidine was not restricted to basal epithelial cells and occurred at any level of the mucosa. Our data showed that the proliferative activity was increased in the mucosa of the human lithiasic gallbladder. To the extent that the labelled cells against the basal membrane in the normal gallbladder represent the epithelial progenitors, our results also indicated modifications in the spatial distribution of labeled cells in the lithiasic gallbladder mucosa. Such concomitant quantitative and qualitative changes in cell kinetics could have a parallel in known precancerous conditions in the digestive tract.