The strain A anti‐p‐azophenylarsonate major crossreactive idiotypic family includes members with no reactivity toward p‐azophenylarsonate
The strain A anti‐p‐azophenylarsonate major crossreactive idiotypic family includes members with no reactivity toward p‐azophenylarsonate
复制标题
A 株抗偶氮苯胂酸主要交叉反应独特型家族包括对偶氮苯胂酸无反应性的成员
DOI：
--
复制
发表时间：
1981
影响因子：
5.4
通讯作者：
V. Sato
中科院分区：
文献类型：
--
作者：
L. Wysocki;V. Sato
未免疫的A/J小鼠的血清含有低水平但可检测到的免疫球蛋白，带有先前描述的诊断A株抗对偶氮苯基亚硝酸盐(ARS)反应的交叉反应独特型(CRI)决定簇。来自非免疫血清的这些分子不能被吸附到与ARs高密度偶联的亲和柱上。在用Ars偶联蛋白广泛免疫后，即使出现了大量的Ars结合CRI，也在相同的低水平上发现了Ars非结合CRI。另一方面，用最初针对Ars结合CRI的鼠抗CRI的单抗免疫，可以诱导高浓度的Ars结合和非Ars结合的CRI免疫球蛋白。用大鼠抗CRI免疫的A/J小鼠产生的三种杂交瘤蛋白与来自三种动物的所有被测抗独特型血清发生反应，但在几种不同的检测中对ARs没有反应。来自同一融合的一个杂交瘤蛋白显示出与Ars结合的能力。
The sera of unimmunized A/J mice contain low but detectable levels of immunoglobulin, bearing previously described cross‐reactive idiotypic (CRI) determinants diagnostic of the strain A anti‐p‐azophenylarsonate (Ars) response. Such molecules from nonimmune sera cannot be adsorbed onto affinity columns coupled to a high density with Ars. After extensive immunization with Ars‐coupled proteins, Ars‐nonbinding CRI is found at the same low level, even though substantial Ars‐binding CRI appears. On the other hand, immunization with a monoclonal rat anti‐CRI, which was originally raised against Ars‐binding CRI, elicits high concentrations of Ars‐binding as well as Ars‐nonbinding CRI immunoglobulin. Three hybridoma proteins produced from an A/J mouse immunized with the rat anti‐CRI react with all tested anti‐idiotypic sera from three species of animals, but show no reactivity toward Ars in several different assays. One hybridoma protein from the same fusion demonstrates Ars‐binding capacity.