Quantitation of microcomputed tomography-imaged ocular microvasculature.
Quantitation of microcomputed tomography-imaged ocular microvasculature.
复制标题
微计算机断层扫描成像的眼部微血管系统的定量。
DOI：
10.1111/j.1549-8719.2009.00009.x
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Atwood RC
中科院分区：
文献类型：
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作者：
Atwood RC
微循环（2010）17，59-68。土井：10.1111/j.1549 - 8719.2009.00009.x摘要目的：使用一种允许三维（3D）图像重建的新技术组合，定量评估新生野生型和VEGF 120-tg小鼠眼中的微血管尺寸。方法：开发了一种新技术组合，用于精确的微血管3D成像，并在新生小鼠眼的玻璃体血管上进行了证明。血管腐蚀铸型用于创建血管网络的稳定复制品，X射线微计算机断层扫描（μCT）用于获得3D图像。内部的计算机辅助图像分析技术，然后进行定量形态学分析的images.Results：与使用这些方法，在血管节段的数量，其直径和体积的玻璃体腔中的血管的差异进行定量在野生型新生小鼠或同窝过表达的不稳定（非肝素结合）亚型的血管内皮生长因子（VEGF 120）从发展中的透镜。该方法具有指导意义，证明了VEGFA 120过表达小鼠的玻璃体血管网络与野生型相比，盲端增加10倍，连接血管节段增加6倍，总玻璃体血管体积增加6倍（0.0314与0.0051 mm 3）。 这些参数不容易通过组织学、超微结构或体视学分析来量化。结论：这里描述的技术的组合提供了器官系统中脉管系统的第一个3D定量表征;即，野生型小鼠和VEGF透镜特异性过表达转基因模型中的新生鼠眼内血管系统。
Microcirculation(2010)17, 59–68. doi: 10.1111/j.1549‐8719.2009.00009.xAbstractPurpose:To quantitatively assess microvascular dimensions in the eyes of neonatal wild‐type and VEGF120‐tg mice, using a novel combination of techniques which permit three‐dimensional (3D) image reconstruction.Methods:A novel combination of techniques was developed for the accurate 3D imaging of the microvasculature and demonstrated on the hyaloid vasculature of the neonatal mouse eye. Vascular corrosion casting is used to create a stable replica of the vascular network and X‐ray microcomputed tomography (μCT) to obtain the 3D images. In‐house computer‐aided image analysis techniques were then used to perform a quantitative morphological analysis of the images.Results:With the use of these methods, differences in the numbers of vessel segments, their diameter, and volume of vessels in the vitreous compartment were quantitated in wild‐type neonatal mice or littermates over‐expressing a labile (nonheparin binding) isoform of vascular endothelial growth factor (VEGF120) from the developing lens. This methodology was instructive in demonstrating that hyaloid vascular networks in VEGFA120over‐expressing mice have a 10‐fold increase in blind‐ended, a six‐fold increase in connected vessel segments, in addition to a sixfold increase (0.0314 versus 0.0051 mm3) in total vitreous vessel volume compared with wild type. These parameters are not readily quantified via histological, ultrastructural, or stereological analysis.Conclusion:The combination of techniques described here provides the first 3D quantitative characterization of vasculature in an organ system; i.e., the neonatal murine intra‐ocular vasculature in both wild‐type mice and a transgenic model of lens‐specific over‐expression of VEGF.