Electroacupuncture promotes neural cell proliferation in vivo through activation of the ERK1/2 signaling pathway
Electroacupuncture promotes neural cell proliferation in vivo through activation of the ERK1/2 signaling pathway
复制标题
电针通过激活ERK1/2信号通路促进体内神经细胞增殖
DOI：
10.3892/ijmm.2014.1702
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发表时间：
2014-06-01
影响因子：
5.4
通讯作者：
Chen, Lidian
中科院分区：
文献类型：
--
作者：
Huang, Jia;Ye, Xiaoqian;Chen, Lidian
本研究旨在探讨电针对细胞增殖的影响及其分子机制。将60只大鼠随机分为5组：假手术对照组（SC）、缺血对照组（IC）、电针组（EA）、电针+DMSO组（艾德）、电针+U 0126组（EU）。除SC组外，其余各组均行大脑中动脉闭塞（MCAO），艾德组或EU组于MCAO前30 min分别注射DMSO或U 0126。增殖细胞核抗原（PCNA）免疫组化染色评价细胞增殖。RT-PCR和Western blot检测细胞周期蛋白cyclin D1、CDK 4、cyclin E、CDK 2、p21和p27的变化及ERK 1/2通路的激活。结果显示：EA组和艾德组PCNA免疫染色阳性细胞数均明显多于IC组（P
The aim of this study was to investigate the effect of electroacupuncture (EA) on cell proliferation and its molecular mechanisms. Sixty rats were randomly divided into 5 groups: sham operation control (SC), ischemia control (IC), EA, EA and DMSO injection (ED), EA and U0126 injection (EU). All the groups, with the exception of SC, underwent middle cerebral artery occlusion (MCAO), and DMSO or U0126 was injected into the rat in the ED or EU group 30 min prior to MCAO. Cell proliferation was evaluated by proliferating cell nuclear antigen (PCNA) immunostaining. The changes of cell cycle proteins (cyclin D1, CDK4, cyclin E, CDK2, p21 and p27) and the ERK1/2 pathway activation were examined by RT-PCR and western blot analysis. The results showed that the positive cell numbers of PCNA immunostaining in the EA and ED groups were more than those in the IC group (P