Effect of dietary supplementation of Bifidobacterium and Lactobacillus strains in Apis mellifera L. against Nosema ceranae
Effect of dietary supplementation of Bifidobacterium and Lactobacillus strains in Apis mellifera L. against Nosema ceranae
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DOI：
10.3920/bm2015.0085
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发表时间：
2016-01-01
影响因子：
5.4
通讯作者：
Di Gioia, D.
中科院分区：
文献类型：
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作者：
Baffoni, L.;Gaggia, F.;Di Gioia, D.
蜜蜂微孢子虫是欧洲蜜蜂广泛分布的一种微孢子虫。影响蜜蜂健康。欧洲联盟对Fumagillin-B（二环己基铵盐）的禁令推动了对预防和控制感染的可持续战略的探索。肠道微生物共生体与脊椎动物和无脊椎动物的肠道系统密切相关，其对宿主健康的影响日益受到人们的关注。特别是，双歧杆菌和乳酸杆菌，它们是蜜蜂消化系统的正常居民，已知通过抗菌代谢物，免疫调节和竞争来保护它们的宿主。在这项工作中，在实验室条件下，在人工感染寄生虫的蜜蜂和未人工感染但证明自然感染率较低的蜜蜂中评估了肠道细菌的饮食补充。在先前分离的双歧杆菌和在这项工作中从健康蜜蜂肠道分离的乳酸杆菌中选择补充的细菌。比较了四种治疗：用糖浆喂养的蜜蜂（CTR）;用含有双歧杆菌和乳酸杆菌的糖浆喂养的蜜蜂（PRO）;感染了N.饲喂含双歧杆菌和乳酸杆菌（NP）的糖浆。从蜜蜂出生的第一天起，将含有或不含微生物的糖浆给予蜜蜂13天。N.在麻醉的5日龄蜜蜂上单独进行宫颈感染。感染后第8天，
Nosema ceranae is a widespread microsporidium of European honeybee Apis mellifera L. affecting bee health. The ban of Fumagillin-B (dicyclohexylammonium salt) in the European Union has driven the search for sustainable strategies to prevent and control the infection. The gut microbial symbionts, associated to the intestinal system of vertebrates and invertebrates and its impact on host health, are receiving increasing attention. In particular, bifidobacteria and lactobacilli, which are normal inhabitants of the digestive system of bees, are known to protect their hosts via antimicrobial metabolites, immunomodulation and competition. In this work, the dietary supplementation of gut bacteria was evaluated under laboratory conditions in bees artificially infected with the parasite and bees not artificially infected but evidencing a low natural infection. Supplemented bacteria were selected among bifidobacteria, previously isolated, and lactobacilli, isolated in this work from healthy honeybee gut. Four treatments were compared: bees fed with sugar syrup (CTR); bees fed with sugar syrup containing bifidobacteria and lactobacilli (PRO); bees infected with N. ceranae spores and fed with sugar syrup (NOS); bees infected with N. ceranae and fed with sugar syrup containing bifidobacteria and lactobacilli (NP). The sugar syrup, with or without microorganisms, was administered to bees from the first day of life for 13 days. N. ceranae infection was carried out individually on anesthetised 5-day-old bees. Eight days after infection, a significant (P