Expression and Regulation of Human CD275 on Endothelial Cells in Healthy and Inflamed Mucosal Tissues
Expression and Regulation of Human CD275 on Endothelial Cells in Healthy and Inflamed Mucosal Tissues
复制标题
DOI：
10.1111/j.1365-3083.2006.01726.x
复制
发表时间：
2006-03
影响因子：
3.7
通讯作者：
P. Youngnak-Piboonratanakit;P. Youngnak-Piboonratanakit;Fumihiko Tsushima;N. Otsuki;Hanna Igarashi;K. Omura
中科院分区：
文献类型：
--
作者：
P. Youngnak-Piboonratanakit;P. Youngnak-Piboonratanakit;Fumihiko Tsushima;N. Otsuki;Hanna Igarashi;K. Omura
CD 275-CD 278共刺激通路是CD 28-B7家族分子参与T细胞介导的免疫应答效应期的新途径。尚未检测健康和疾病状态下口腔粘膜中CD 275的表达。我们产生了抗人CD 275的单克隆抗体，并研究了其在培养的组织细胞系和口腔粘膜组织中的表达和调节。单核细胞上的CD 275被白细胞介素-4有效上调，而干扰素-γ消除了这种作用。培养的内皮细胞（EC）上的CD 275被肿瘤坏死因子-α迅速增强。在健康口腔粘膜中，在上皮内的角质形成细胞、朗格汉斯细胞或上皮内淋巴细胞上或在上皮下的间质树突状细胞或淋巴细胞上未检测到CD 275。在正常口腔黏膜中，结缔组织EC上的CD 275呈组成性表达，而在口腔扁平苔藓中，EC上的CD 275表达上调或下调。大约20%的T细胞内发现浸润单核细胞在subepithelium表达高水平的CD 278受体。淋巴细胞和非淋巴细胞上的CD 275受多种细胞因子的正或负调节。我们的研究结果表明，EC上的CD 275参与受体阳性效应T细胞向炎症组织的募集或外渗。
The CD275‐CD278 costimulatory pathway is a new pathway for CD28‐B7 family molecules involved in the effector phase of T‐cell‐mediated immune responses. Expression of CD275 in oral mucosa at healthy and disease states has not been examined. We generated monoclonal antibodies against human CD275 and investigated its expression and regulation in cultured tissue cell lines and oral mucosal tissues. CD275 on monocytes was efficiently upregulated by interleukin‐4, while interferon‐γ abrogated this effect. CD275 on cultured endothelial cells (EC) was rapidly enhanced by tumour necrosis factor‐α. In healthy oral mucosa, CD275 was not detected on keratinocytes, Langerhans cells or intraepithelial lymphocytes within the epithelium or on interstitial dendritic cells or lymphocytes in the subepithelium. Constitutive expression of CD275 on EC in the connective tissues was observed in healthy mucosa, but CD275 expression on EC in oral lichen planus was either upregulated or downregulated. Approximately 20% of the T cells found within infiltrating mononuclear cells in the subepithelium expressed high levels of the CD278 receptor. CD275 on lymphoid and nonlymphoid cells is positively or negatively regulated by various cytokines. Our results suggest that CD275 on EC is involved in the recruitment or extravasation of receptor‐positive effector T cells into inflamed tissues.