Selective downregulation of VEGF-A165, VEGF-R1, and decreased capillary density in patients with dilative but not ischemic cardiomyopathy
Selective downregulation of VEGF-A165, VEGF-R1, and decreased capillary density in patients with dilative but not ischemic cardiomyopathy
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DOI：
10.1161/01.res.87.8.644
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发表时间：
2000-10-13
影响因子：
20.1
通讯作者：
Aharinejad, S
中科院分区：
文献类型：
--
作者：
Abraham, D;Hofbauer, R;Aharinejad, S
心肌病(CM)包括一组不同的疾病，包括缺血性(ICM)和扩张型(DCM)。原发性扩张型心肌病的发病机制尚不清楚。最近在小鼠身上的研究表明，血管内皮生长因子(VEGF)参与了ICM。目前尚不清楚血管内皮生长因子在人类CHI中是否发挥作用。我们采用实时定量聚合酶链式反应和Western blotting方法检测了终末期DCM和ICM患者及健康人心脏组织中血管内皮生长因子及其受体的mRNA和蛋白表达。用透射电子显微镜计算心肌活检中的毛细血管数、心肌细胞面积和胶原蛋白。除血管内皮生长因子C外，扩张型心肌病组血管内皮生长因子A(165例)、血管内皮生长因子A(189例)、血管内皮生长因子B(B)的mRNA转录水平及蛋白表达水平均较正常对照组下调(P
Cardiomyopathy (CM) comprises a heterogeneous group of diseases, including ischemic (ICM) and dilative (DCM) forms. The pathogenesis of primary DCM is not clearly understood. Recent studies in mice show that vascular endothelial growth factor (VEGF) is involved in ICM. Whether VEGF plays a role in human Chi is unknown. We examined the mRNA and protein expression of VEGF and its receptors in hearts of patients with end-stage DCM and ICM and in healthy individuals using real-time polymerase chain reaction and Western blotting. Number of capillaries, area of myocytes, and collagen were calculated in cardiac biopsies using transmission electron microscopy. In DCM, except for VEGF-C, mRNA transcript levels of VEGF-A(165), VEGF-A(189), and VEGF-B and the protein level of VEGF-A and VEGF-R-1 were downregulated compared with controls (P