Organoid Models of Human Gastrointestinal Development and Disease.
Organoid Models of Human Gastrointestinal Development and Disease.
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DOI：
10.1053/j.gastro.2015.12.042
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发表时间：
2016-05
期刊：
影响因子：
29.4
通讯作者：
Spence JR
中科院分区：
文献类型：
--
作者：
Dedhia PH;Bertaux-Skeirik N;Zavros Y;Spence JR
我们已经大大提高了我们在体外培养各种组织衍生和多能干细胞衍生胃肠道（GI）组织的能力。这些系统，广泛地称为类器官，使该领域远离了几十年来在GI研究中使用的通常非生理性的转化细胞系。类器官来源于初生组织，具有长期生长的能力。它们包含不同水平的细胞复杂性和与天然器官系统的生理相似性。我们回顾了组织来源的和多能干细胞来源的肠、胃、食管、肝和胰腺类器官研究的最新发现。这些研究为GI发育，组织稳态和疾病提供了重要的见解，并可用于开发个性化药物。
We have greatly advanced our ability to grow a diverse range of tissue-derived and pluripotent stem cell-derived gastrointestinal (GI) tissues in vitro. These systems, broadly referred to as organoids, have allowed the field to move away from the often non-physiologic, transformed cell lines that have been used for decades in GI research. Organoids are derived from primary tissues and have the capacity for long-term growth. They contain varying levels of cellular complexity and physiologic similarity to native organ systems. We review the latest discoveries from studies of tissue-derived and pluripotent stem cell-derived intestinal, gastric, esophageal, liver, and pancreatic organoids. These studies have provided important insights into GI development, tissue homeostasis, and disease and might be used to develop personalized medicines.