EFFECT OF COLD PRESERVATION ON LYMPHOCYTE MIGRATION INTO PERIPHERAL-NERVE ALLOGRAFTS IN SHEEP
EFFECT OF COLD PRESERVATION ON LYMPHOCYTE MIGRATION INTO PERIPHERAL-NERVE ALLOGRAFTS IN SHEEP
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DOI：
10.1097/00007890-199307000-00029
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发表时间：
1993-07-01
期刊：
影响因子：
6.2
通讯作者：
HAY, JB
中科院分区：
文献类型：
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作者：
HARE, GMT;EVANS, PJ;HAY, JB
通过检测淋巴细胞在新鲜和保存的同种异体周围神经移植物中的迁移，评价低温保存和冻融预处理对同种异体神经移植物局部免疫反应的影响。远交母羊接受多个1.5厘米皮下异位腓神经自体移植物，新鲜同种异体移植物，和预处理的同种异体移植物，植入同一受体。通过静脉注射自体铟-111标记的淋巴细胞，在第7天研究淋巴细胞迁移。再循环3小时后，通过γ计数器定量淋巴细胞迁移到移植物组织中（cpm/g，平均值+/- SEM）。淋巴细胞进入新鲜同种异体神经移植物（21，623 +/- 3783）比自体移植物平均增加了9.4倍（2918 +/- 377，P
Lymphocyte migration into fresh and preserved peripheral nerve allografts was quantitated to assess the effect of cold preservation and freeze-thawing pretreatment on the local immunological response to nerve allografts. Out-bred ewes received multiple 1.5-cm subcutaneous heterotopic peroneal nerve autografts, fresh allografts, and pretreated allografts, implanted within the same recipient. Lymphocyte migration was studied at 7 days by injecting autologous indium-111-labeled lymphocytes intravenously. After 3 hr of recirculation, lymphocyte migration into graft tissue was quantitated by a gamma counter (cpm/g, mean +/- SEM). Lymphocyte traffic into fresh nerve allografts (21,623 +/- 3783) increased an average 9.4-fold over the autograft value (2918 +/- 377, P