Vascular remodeling in ApoE-deficient mice: Diet dependent modulation after carotid ligation
Vascular remodeling in ApoE-deficient mice: Diet dependent modulation after carotid ligation
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DOI：
10.1016/j.atherosclerosis.2008.08.025
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发表时间：
2009-05-01
期刊：
影响因子：
5.3
通讯作者：
Weissen-Plenz, G.
中科院分区：
文献类型：
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作者：
Eschert, H.;Sindermann, J. R.;Weissen-Plenz, G.
血管重塑受创伤和致动脉粥样硬化因素如胆固醇的影响。胆固醇对血管壁结构有直接影响。在这项研究中，我们评估了血管创伤和胆固醇治疗对颈动脉结扎的ApoE缺陷小鼠的血管重塑和斑块完整性的影响。4周后，制备左（未结扎）和右（结扎）颈动脉。为了研究血管重塑，通过从Verhoff-van Gieson染色上的周长测量计算面积，在形态学上评价血管面积。通过组织学染色和免疫组化分析斑块的细胞和结构特征，规律饮食下总血管面积减少35%（p
Vascular remodeling is influenced by trauma and proatherogenic factors such as cholesterol. It has been shown that cholesterol exerts a direct effect on vessel wall structure. In this study we evaluated the effects of vascular trauma and cholesterol treatment on vascular remodeling and plaque integrity in carotid ligated ApoE-deficient mice.The right carotid artery was ligated in mice fed regular chow or cholesterol and fat containing diet. After 4 weeks left (non-ligated) and right (ligated) carotids were prepared. For studying vascular remodeling the vascular areas were evaluated morphometrically by calculating the areas from circumference measurements on Verhoff-van Gieson stains. The cellular and structural features of the plaque were analyzed by histological staining and immunohistochemistry.Under regular chow total vessel area decreased by 35% (p