Generation of trophoblast stem cells.
Generation of trophoblast stem cells.
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DOI：
10.1007/978-1-62703-011-3_3
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Golding, Michael C
中科院分区：
文献类型：
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作者：
Golding, Michael C
胚胎干细胞和胚外干细胞的分离和培养为研究建立和维持谱系特异性、单等位基因基因表达模式的分子过程提供了巨大的机会。本章描述了从小鼠胚泡期胚胎中分离培养滋养外胚层干细胞.利用这种强大的体外系统，科学家们现在可以开始梳理出导致胎盘印记基因表达模式的表观遗传过程，并开始更好地了解这些基因在发育和疾病中的作用。
The isolation and culture of both embryonic and extraembryonic stem cells provide an enormous opportunity to study the molecular processes that establish and maintain lineage-specific, monoallelic patterns of gene expression. This chapter describes the isolation an culture of trophectoderm stem cells from mouse blastocyst stage embryos. Using this powerful in vitro system, scientists can now begin to tease apart the epigenetic processes that result in placental patterns of imprinted gene expression and begin to better understand the role these genes play in development and disease.