Chronic in vivo nerve electrical recordings of Aplysia californica using a boron-doped polycrystalline diamond electrode
Chronic in vivo nerve electrical recordings of Aplysia californica using a boron-doped polycrystalline diamond electrode
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DOI：
10.1016/j.diamond.2009.08.006
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发表时间：
2010-02-01
影响因子：
4.1
通讯作者：
Martin, Heidi B.
中科院分区：
文献类型：
--
作者：
Halpern, Jeffrey M.;Cullins, Miranda J.;Martin, Heidi B.
定制的金刚石电极在体内，被动电神经记录的开发和植入一个完整的，自由行为的加利福尼亚州。相对于传统的神经记录电极材料，金刚石电极可能具有用于电记录的优势，因为其化学稳定性、较高的信噪比和减少的生物污染。例如，在失智症中用于慢性细胞外记录的薄不锈钢电极通常在体内持续大约七天。我们开发了第一代金刚石电极，带有柔性电极导线，可承受Ablasia的复杂运动，并可与植入的不锈钢电极进行直接比较。采用热灯丝化学气相沉积法在钨丝衬底上生长了掺硼多晶金刚石。将导丝预成形为钩（用于神经记录）或环（用于接地导丝），并掩蔽仅2-3 mm的导丝上的选择性生长。在金刚石生长之后，去除线材基材的未涂覆部分，并将完整的金刚石涂覆的钩或环附接到具有导电环氧树脂的不锈钢电引线。环氧树脂固化后，将电极绝缘后植入动物体内，将金刚石和不锈钢电极细胞外贴附于A.加利福尼亚州。这两个电极彼此相邻植入，以便记录几乎同步。在植入后长达9天的进食行为期间，在金刚石钩电极上成功地获得了体内记录。电极在动物体内和取出后总共保持完整28天。(C)2009年由Elsevier B. V.出版
Custom diamond electrodes for in vivo, passive electrical nerve recording were developed and implanted in an intact, freely behaving Aplysia californica. A diamond electrode may have advantages for electrical recording because of its chemical stability, higher signal-to-noise ratio, and reduced biological fouling relative to traditional nerve-recording electrode materials. For example, thin stainless steel electrodes used for chronic extracellular recording in Aplysia typically last on the order of seven days in vivo. We have developed a first-generation diamond electrode with a flexible lead to withstand the complicated movements of Aplysia and to enable direct comparison with implanted stainless steel electrodes. Boron-doped polycrystalline diamond was grown onto tungsten wire substrates by hot filament chemical vapor deposition. The wires were pre-shaped into a hook (for nerve recording) or a loop (for a ground wire) and masked for selective growth on only 2-3 mm of the wire. After diamond growth, the uncoated portion of the wire substrates was removed and the intact diamond-coated hook or loop attached to a stainless steel electrical lead with conductive epoxy. After epoxy curing, these electrodes were insulated and implanted in the animal.The diamond and stainless steel electrodes were attached extracellularly to buccal nerve 2, a primary nerve for the feeding behavior of A. californica. The two electrodes were implanted adjacent to each other so that the recordings would be nearly synchronous. In vivo recordings were successfully obtained on a diamond hook electrode during a feeding behavior for up to 9 days after the implantation. The electrode remained intact for a total of 28 days in the animal and upon retrieval. (C) 2009 Published by Elsevier B.V.