Interspecies Transmission, Genetic Diversity, and Evolutionary Dynamics of Pseudorabies Virus
Interspecies Transmission, Genetic Diversity, and Evolutionary Dynamics of Pseudorabies Virus
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DOI：
10.1093/infdis/jiy731
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发表时间：
2019-06-01
影响因子：
6.4
通讯作者：
Su, Shuo
中科院分区：
文献类型：
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作者：
He, Wanting;Auclert, Lisa Zoe;Su, Shuo
背景伪狂犬病病毒（PRV）引起猪的Aujeszky病，并可传播给其他哺乳动物，包括人类。本研究系统地研究了PRV的种间传播和进化史。通过对伪狂犬病病毒的进化动力学、选择性和结构生物学等方面的综合分析，总结了伪狂犬病病毒的系统发育和适应性进化。PRV可分为2个主要分支，分支间和分支内重组频繁。进化枝2.2（变异PRV）是目前世界上最流行的基因型，最常参与跨物种传播事件（包括人类）。我们还发现，2011年以来，2.2分支的种群规模有所增加，2011 - 2016年有效繁殖数均>1，表明PRV可能仍在猪群中流行，在中国仍存在与跨种传播相关的风险。值得注意的是，我们鉴定了一些重要的糖蛋白gB、gC、gD和gE中的氨基酸位点，这些位点可能与PRV对新宿主的适应和对疫苗的免疫逃逸有关。我们的研究提供了重要的遗传学见解的种间传播和进化的PRV内和不同的主机之间，保证额外的监测。
Background. Pseudorabies virus (PRV) causes Aujeszky's disease in pigs and can be transmitted to other mammals, including humans. In the current study, we systematically studied the interspecies transmission and evolutionary history of PRV.Methods. We performed comprehensive analysis on the phylodynamics, selection, and structural biology to summarize the phylogenetic and adaptive evolution of PRV based on all available full-length and major glycoprotein sequences.Results. PRV can be divided into 2 main clades with frequent interclade and intraclade recombination. Clade 2.2 (variant PRV) is currently the most prevalent genotype worldwide, and most commonly involved in cross-species transmission events (including humans). We also found that the population size of clade 2.2 has increased since 2011, and the effective reproduction number was >1 from 2011 to 2016, indicating that PRV may be still circulating in swine herds and is still a risk in relation with cross-species transmission in China. Of note, we identified amino acid sites in some important glycoproteins gB, gC, gD, and gE that may be associated with PRV adaptation to new hosts and immune escape to vaccines.Conclusions. Our study provides important genetic insight into the interspecies transmission and evolution of PRV within and between different hosts that warrant additional surveillance.