Plasma membrane proteome analyses of Arabidopsis suspension cultured cells (T87 line) associated with freezing tolerance and growth phase.
Plasma membrane proteome analyses of Arabidopsis suspension cultured cells (T87 line) associated with freezing tolerance and growth phase.
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与冷冻耐受性和生长期相关的拟南芥悬浮培养细胞（T87 系）的质膜蛋白质组分析。
DOI：
10.1016/j.jprot.2019.103528
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发表时间：
2020
影响因子：
3.3
通讯作者：
Uemura M.
中科院分区：
文献类型：
--
作者：
Li B;Takahashi D;Kawamura Y;Uemura M.
冷驯化（CA）和脱落酸（阿坝）处理对拟南芥悬浮培养细胞的抗冻性有影响：处理后，滞后期和对数期细胞的抗冻性增强，而静止期细胞的抗冻性反而下降。为了进一步表征细胞对这些处理的不同反应，使用无标记蛋白质定量技术分析了质膜（PM）蛋白质组。841个蛋白质的表达在生长期、CA和/或阿坝处理过程中发生变化。其中，392种蛋白质在生长阶段进展期间在细胞中的丰度发生变化，并被分类为各种功能组，这表明PM的生理特性随生长阶段而变化。CA和ABA反应蛋白的比较表明，阿坝是PM蛋白质组响应CA的重要信号之一，但PM蛋白质对CA的反应需要多种信号。阿坝在CA过程中的参与随着生长期的进展而减少。以上结果表明，随着生长阶段的进展，PM蛋白质组的动态变化影响PM蛋白质组对CA和阿坝的反应，这可能影响拟南芥悬浮培养细胞（T87系）在冷驯化（CA）或脱落酸（阿坝）处理后的抗冻能力的差异。然而，质膜（PM）蛋白质组谱是否不同的生长阶段之间的差异，并与生长阶段依赖的抗冻性的差异尚未阐明。本研究以紫茎泽兰悬浮培养细胞为研究对象，研究了紫茎泽兰悬浮培养细胞在不同生长阶段的PM蛋白质组学特征。PM蛋白质组的特征随着生长阶段的进展而发生显著变化，阿坝是CA期间PM蛋白质变化的重要信号。结果还表明，PM蛋白对CA的反应需要多个信号才能完成。因此，PM蛋白质组随着生长期的推进而发生的动态变化影响了PM蛋白质组对CA和阿坝的反应，这可能导致培养细胞的抗冻性差异。
Cold acclimation (CA) and abscisic acid (ABA) treatments affected freezing tolerance ofArabidopsis thalianasuspension-cultured cells: cells at the lag and log phases increased their freezing tolerance but cells at the stationery phase rather decreased after the treatments. To further characterize the differential responses of the cells to these treatments depending on growth phase, plasma membrane (PM) proteome were analyzed using label-free protein quantification technology. Abundance of 841 proteins changed during the progression of growth phase, CA and/or ABA treatment. Among them, 392 proteins changed in their abundance in cells during growth phase progression and were categorized into various functional groups, suggesting changes in physiological characteristics of the PM depending on the growth phase. Comparison of CA- and ABA-responsive proteins indicated that ABA is one of the important signals for PM proteome changes in response to CA, but multiple signals are required for the response of PM proteins to CA. Involvement of ABA in the CA process diminished with the progression of growth phase. Taken together, the results suggest that dynamic alterations of the PM proteome with the progression of growth phase influence the PM proteome responses to CA and ABA, which may effect the difference in freezing tolerance capability.SignificanceAfter cold acclimation (CA) or abscisic acid treatment (ABA),Arabidopsis thalianasuspension-cultured cells (T87 line) exhibited freezing tolerance capability dependent on the cell growth phase. However, whether the plasma membrane (PM) proteome profile differs among growth phases and the differences are associated with growth-phase-dependent freezing tolerance have not been elucidated. In the present study, PM proteomics was conducted in association with CA and ABA treatment ofArabidopsissuspension-cultured cells at three growth phases. Characteristics of the PM proteome changed considerably with progression of the growth phase and ABA was indicated to be an important signal for PM protein changes during CA. The results also revealed that multiple signals are required to complete the response of PM proteins to CA. Therefore, dynamic alterations of the PM proteome with the advance of the growth phase influence the responses of PM proteome to CA and ABA, which may result in the differences in freezing tolerance of the cultured cells.