Imaging macrophages and the apoptosis of granulocytes in a rodent model of subacute and chronic abscesses with radiolabeled monocyte chemotactic peptide-1 and annexin V
Imaging macrophages and the apoptosis of granulocytes in a rodent model of subacute and chronic abscesses with radiolabeled monocyte chemotactic peptide-1 and annexin V
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DOI：
10.1007/s002590100572
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发表时间：
2001-09-01
期刊：
影响因子：
--
通讯作者：
Strauss, HW
中科院分区：
文献类型：
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作者：
Blankenberg, FG;Tait, JF;Strauss, HW
单核细胞/巨噬细胞 (Mos) 是亚急性和慢性炎症中的主要细胞类型，被单核细胞趋化肽-1 (MCP-1) 吸引并激活。 Mos 通过诱导细胞凋亡和吞噬衰老（耗尽）和旁观者（多余）粒细胞来促进炎症的消退。我们希望确定选择性结合 Mos 的 MCP-1 是否可以用于亚急性和慢性炎症的成像。我们还试图用 Technetium-99m 标记的膜联蛋白 V（凋亡细胞的标记物）对这些病变内的粒细胞凋亡进行成像。通过将松节油深部肌肉注射到 45 只 12 周龄的雄性 Sprague-Dawley 大鼠的右大腿中，诱导无菌炎症。然后在松节油处理后1-14天尾静脉注射37-148MBq(1-4mCi)的Tc-99m标记的MCP-I或膜联蛋白V后1小时对四至六只动物进行成像。图像分析显示，发炎大腿中的 MCP-I 和膜联蛋白 V 活性均显着高于对照大腿（分别为 165%-290% 和 188%-313%；P
Monocytes/macrophages (Mos), the predominant cell types in subacute and chronic inflammation, are attracted to and activated by monocyte chemotactic peptide-1 (MCP-1). Mos promote the resolution of inflammation through the induction of apoptosis and phagocytosis of senescent (spent) and bystander (superfluous) granulocytes. We wished to determine whether MCP-1, which selectively binds to Mos, could be used to image subacute and chronic inflammation. We also sought to image granulocyte apoptosis within these lesions with technetium-99m labeled annexin V, a marker of apoptotic cells. Sterile inflammation was induced in 45 12-week-old male Sprague-Dawley rats by deep intramuscular injection of turpentine into the right thigh. Groups of four to six animals were then imaged I h after tail vein injection of 37-148 MBq (1-4 mCi) of Tc-99m-labeled MCP-I or annexin V 1-14 days after turpentine treatment. Image analysis showed significantly greater activity of both MCP-I and annexin V in inflamed thighs than in control thighs (165%-290% and 188%-313%, respectively; P