BODIPY Fluorescent Chemosensor for Cu2+ Detection and Its Applications in Living Cells: Fast Response and High Sensitivity
BODIPY Fluorescent Chemosensor for Cu2+ Detection and Its Applications in Living Cells: Fast Response and High Sensitivity
复制标题
用于 Cu2 检测的 BODIPY 荧光化学传感器及其在活细胞中的应用：快速响应和高灵敏度
DOI：
10.1007/s10895-014-1360-9
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发表时间：
2014-05-01
影响因子：
2.7
通讯作者：
Jing, Xiabin
中科院分区：
文献类型：
--
作者：
Quan, Li;Sun, Tingting;Jing, Xiabin
铜是人体器官正常运作和代谢过程中必不可少的微量元素。然而，它在体内的过量和不足都可能导致不良的健康影响。合成了一种含2，2 '-联吡啶基的BODIPY荧光化学剂量计，在温和条件下选择性检测Cu ~（2+）。该BODIPY对水溶液中的Cu 2+具有快速响应（近似1 min）和高灵敏度，这是由于光诱导电子从荧光团的激发态转移到与Cu 2+络合的联吡啶单元。MALDI-TOF质谱揭示的荧光猝灭机制表明，一个Cu 2+可以与两个BODIPY分子配位，并且这种配位是可逆的。这种简单的BODIPY染料也可用于检测活细胞中的Cu ~（2+）。这项工作有助于将BODIPY的潜在应用扩展到生物和环境领域。
Copper is an essential trace element for the proper functioning of organ and metabolic process in humans. However, both its excess and deficiency in the body can result in adverse health effects. A BODIPY containing 2,2'-bipyridyl group was synthesized and used as a fluorescent chemodosimeter for selective Cu2+ detection in mild condition. This BODIPY shows fast response (similar to 1 min) and high sensitivity for Cu2+ in aqueous solution due to the photoinduced electron transfer from the excited state of fluorophore to the bipyridyl unit complexed to Cu2+. The fluorescence quenching mechanism revealed by MALDI-TOF Mass spectra showed one Cu2+ could coordinate with two BODIPY molecules, and this coordination is reversible. This simple BODIPY dyes also could be used for sensing the Cu2+ in living cell. This work contributes to extend the potential applications of BODIPY to the biological and environmental areas.