An Ac transposon system based on maize chromosome 4S for isolating long-distance-transposed Ac tags in the maize genome
An Ac transposon system based on maize chromosome 4S for isolating long-distance-transposed Ac tags in the maize genome
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DOI：
10.1007/s10709-010-9526-0
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发表时间：
2010-11
期刊：
影响因子：
1.5
通讯作者：
Fei Wang;Zhaoying Li;Jun Fan;Pengfei Li;Wei-wei Hu;Gang Wang;Zhengkai Xu;R. Song
中科院分区：
文献类型：
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作者：
Fei Wang;Zhaoying Li;Jun Fan;Pengfei Li;Wei-wei Hu;Gang Wang;Zhengkai Xu;R. Song
转座子标签是基因分离和功能研究的重要工具。在玉米中，已经发展了几种转座子标记系统，主要使用激活/解离(Ac/DS)和诱变系统。在这里，我们建立了另一个基于Ac的转座子系统，供体Ac与玉米染色体4S上的Sugary1(Su1)紧密连锁。根据负剂量效应检测到新的转位Ac(tr-acs)，并通过简单的回交方案从供者Ac中鉴定和分离出远距离转位的Ac事件。在本研究中，我们鉴定了208个独立的长距离易位基因。在玉米基因组中分离并定位了31个这三个位点的侧翼序列。正如以前的研究发现的那样，基因序列中插入了一种参照插入的基因。其分布不是随机的。在我们的研究中，tr-Acs被参照地转座到1，2，9和10号染色体上。我们讨论了来自Acs的tr-Acs的优先分布。我们的系统是对另外两个基于Ac的玉米区域突变系统的补充，这些系统的组合使用将在玉米基因组中实现均匀和高密度的元素分布，用于功能基因组学研究。
Transposon tagging is an important tool for gene isolation and functional studies. In maize, several transposon-tagging systems have been developed, mostly usingActivator/Dissociation(Ac/Ds) and Mutator systems. Here, we establish anotherAc-based transposon system with the donorActightly linked withsugary1(su1) on maize chromosome 4S. Newly transposedAc(tr-Acs) were detected based on a negative dosage effect, and long-distance-transposedAcevents were identified and isolated from the donorAcby a simple backcross scheme. In this study, we identified 208 independent long-distance-transposedAclines. Thirty-one flanking sequences of thesetr-Acswere isolated and localized in the maize genome. As found in previous studies, thetr-Acspreferentially inserted into genic sequences. The distribution oftr-Acsis not random. In our study, thetr-Acspreferentially transposed into chromosomes 1, 2, 9 and 10. We discuss the preferential distribution oftr-AcsfromAcsystems. Our system is complementary to two otherAc-based regional-mutagenesis systems in maize, and the combined use of these systems will achieve an even and high-density distribution ofAcelements throughout the maize genome for functional-genomics studies.