Global transcriptional profiling of a cold-tolerant rice variety under moderate cold stress reveals different cold stress response mechanisms.
Global transcriptional profiling of a cold-tolerant rice variety under moderate cold stress reveals different cold stress response mechanisms.
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DOI：
10.1111/ppl.12291
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发表时间：
2015-07
影响因子：
6.4
通讯作者：
Junliang Zhao;Shaohong Zhang;Tifeng Yang;Zichong Zeng;Zhanghui Huang;Qing Liu;Xiaofei Wang;J. Leach;H. Leung;B. Liu
中科院分区：
文献类型：
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作者：
Junliang Zhao;Shaohong Zhang;Tifeng Yang;Zichong Zeng;Zhanghui Huang;Qing Liu;Xiaofei Wang;J. Leach;H. Leung;B. Liu
已经在包括水稻在内的植物中进行了严重冷胁迫（4°C）下的基因表达谱分析。然而，稻苗在自然环境下经常暴露于较温和的冷胁迫下。为了了解水稻对轻度寒冷胁迫的反应，在对耐冷粳稻品种丽江新团黑谷（LTH）的基因表达进行全基因组分析之前，采用中等低温（8°C）进行冷处理。在中等冷应激期间的四个时间点总共发现了 5557 个差异表达基因（DEG）。 DEG 和差异表达的转录因子基因根据其表达情况分为两组，表明对冷应激的两阶段反应以及转录因子在应激反应调节中的决定性作用。本研究首次报道了冷应激下 OsDREB2A 的诱导。在抗氧化酶基因中，谷胱甘肽过氧化物酶（GPX）和谷胱甘肽S-转移酶（GST）表达上调，表明谷胱甘肽系统可能是LTH中主要的活性氧（ROS）清除剂。生长素、脱落酸（ABA）和水杨酸（SA）信号转导途径中基因表达的变化意味着它们参与冷应激反应。 ABA反应基因的诱导和DEG中富集顺式元件的检测表明ABA信号通路在冷应激反应中起主导作用。我们的结果表明，水稻对冷胁迫的反应随施加的特定温度和水稻基因型的不同而变化。
Gene expression profiling under severe cold stress (4°C) has been conducted in plants including rice. However, rice seedlings are frequently exposed to milder cold stresses under natural environments. To understand the responses of rice to milder cold stress, a moderately low temperature (8°C) was used for cold treatment prior to genome-wide profiling of gene expression in a cold-tolerant japonica variety, Lijiangxintuanheigu (LTH). A total of 5557 differentially expressed genes (DEGs) were found at four time points during moderate cold stress. Both the DEGs and differentially expressed transcription factor genes were clustered into two groups based on their expression, suggesting a two-phase response to cold stress and a determinative role of transcription factors in the regulation of stress response. The induction of OsDREB2A under cold stress is reported for the first time in this study. Among the anti-oxidant enzyme genes, glutathione peroxidase (GPX) and glutathione S-transferase (GST) were upregulated, suggesting that the glutathione system may serve as the main reactive oxygen species (ROS) scavenger in LTH. Changes in expression of genes in signal transduction pathways for auxin, abscisic acid (ABA) and salicylic acid (SA) imply their involvement in cold stress responses. The induction of ABA response genes and detection of enriched cis-elements in DEGs suggest that ABA signaling pathway plays a dominant role in the cold stress response. Our results suggest that rice responses to cold stress vary with the specific temperature imposed and the rice genotype.