Salmonella electrode for screening mutagens.
Salmonella electrode for screening mutagens.
复制标题
用于筛选诱变剂的沙门氏菌电极。
DOI：
10.1021/ac00248a015
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发表时间：
1982
影响因子：
7.4
通讯作者：
S. Suzuki
中科院分区：
文献类型：
--
作者：
I. Karube;T. Nakahara;T. Matsunaga;S. Suzuki
诱变剂的初步筛选在10 h内完成，使用由氧电极和保留鼠伤寒沙门菌逆转录物的膜过滤器组成的微生物电极系统。目前的下降与诱变剂浓度有关。AF-2的最低可测浓度为0.001 pg mL" 1。该微生物电极系统还被应用于检测其他化学诱变剂，如Af-methyl-Af'- ri硝基- af - nittrotrosoguanidne， nittrofurazone methyl methyl磺酸和ethyl methyl磺酸。致癌物的致突变活性最近已在大量病例中得到证实(1)。化学物质的致突变性和致癌性之间存在着高度的相关性，这是显而易见的。微生物系统的使用对致突变化学品的调查是重要的。近年来，许多用于检测各种类型诱变剂的微生物方法已经被开发出来。利用鼠伤寒沙门菌（2,3）或大肠杆菌(4)的微生物还原试验已被用于化学致癌物的筛选试验。提出了一种利用枯草芽孢杆菌筛选化学成分的方法“Rec-assay”
Preliminary screening of mutagens was completed within 10 h by using a microbial electrode system composed of an oxygen electrode and a membrane filter retaining Salmonella typhimurium revertant. The current decrease was correlated with mutagen concentrations. The minimum measurable concentration for AF-2 was 0.001 pg mL" 1. The microbial electrode system was applied to the detection of other chemical mutagens such as Af-methyl-Af'-riitro-Af-nltroso-guanidlne, nltrofurazone methyl methanesulfonate, and ethyl methanesulfonate.The mutagenic activity of carcinogens has recently been confirmed in a great number of cases (1). The existence of a high correlation between the mutagenicity and carcinogenicity of chemicals is now evident. The use of the microbial system is important for a survey of mutagenic chemicals. Recently, a number of microbial methods for detecting various types of mutagens have been developed. Microbial reversion assay using Salmonella typhimurium (2, 3) or Escherichia coli (4) have been employed for screening tests of chemical carcinogens. A method named “Rec-assay” using Bacillus subtilis has also been proposed for screening chemical mu-