Dmrt1 expression is regulated by follicle-stimulating hormone and phorbol esters in postnatal Sertoli cells.
Dmrt1 expression is regulated by follicle-stimulating hormone and phorbol esters in postnatal Sertoli cells.
复制标题
DOI：
10.1210/endo.142.3.8021
复制
发表时间：
2001-03
期刊：
影响因子：
4.8
通讯作者：
Jiang kai Chen;L. Heckert
中科院分区：
文献类型：
--
作者：
Jiang kai Chen;L. Heckert
dmrt 1是最近描述的基因，其仅在睾丸中表达，并且是出生后睾丸分化所必需的。在这里，我们描述的表达Dmrt 1在出生后大鼠睾丸和Sertoli细胞。RNA酶保护分析被用来检查Dmrt 1信使RNA（mRNA）水平在完整的睾丸在出生后的发展和支持细胞在各种培养条件下的原代培养。我们发现，Dmrt 1 mRNA水平显着上升，出生后约10天开始，并保持升高，直到出生后第三周。此后，mRNA水平下降，与睾丸中生殖细胞的增殖同时发生。在新鲜分离的Sertoli细胞中，Dmrt 1 mRNA水平是稳健的，但当细胞置于培养物中24 h时显著降低。用FSH或8-溴-cAMP处理支持细胞导致Dmrt 1 mRNA水平显著升高。这种cAMP反应对转录抑制剂放线菌素D治疗敏感，但对翻译抑制剂放线菌酮不敏感。Dmrt 1 mRNA的cAMP依赖性升高也需要激活蛋白激酶A，因为mRNA诱导对抑制剂H89敏感。研究还表明，Dmrt 1的表达受到佛波醇酯（PMA）的抑制，但只有适度的影响，血清。
Dmrt1 is a recently described gene that is expressed exclusively in the testis and is required for postnatal testis differentiation. Here we describe the expression of Dmrt1 in postnatal rat testis and Sertoli cells. RNase protection analysis was used to examine Dmrt1 messenger RNA (mRNA) levels in intact testis during postnatal development and in primary cultures of Sertoli cells under various culture conditions. We show that Dmrt1 mRNA levels rise significantly beginning approximately 10 days after birth and remain elevated until after the third postnatal week. Thereafter, mRNA levels drop coincident with the proliferation of germ cells in the testis. In freshly isolated Sertoli cells, Dmrt1 mRNA levels were robust but decreased significantly when the cells were placed in culture for 24 h. Treatment of Sertoli cells with either FSH or 8-bromo-cAMP resulted in a significant rise in Dmrt1 mRNA levels. This cAMP response was sensitive to treatment with the transcriptional inhibitor actinomycin D but not to the translational inhibitor cycloheximide. The cAMP-dependent rise in Dmrt1 mRNA also required activation of protein kinase A, as mRNA induction was sensitive to the inhibitor H89. Studies also show that Dmrt1 expression was inhibited by phorbol esters (PMA) but only modestly effected by serum.