INDUCTION OF MAMMARY CARCINOMAS IN RATS BY NITROSO-METHYLUREA INVOLVES MALIGNANT ACTIVATION OF H-RAS-1 LOCUS BY SINGLE POINT MUTATIONS
INDUCTION OF MAMMARY CARCINOMAS IN RATS BY NITROSO-METHYLUREA INVOLVES MALIGNANT ACTIVATION OF H-RAS-1 LOCUS BY SINGLE POINT MUTATIONS
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DOI：
10.1038/306658a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
BARBACID, M
中科院分区：
文献类型：
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作者：
SUKUMAR, S;NOTARIO, V;BARBACID, M
用亚硝基甲基脲诱发50日龄Buf/N雌性大鼠9例乳腺癌，均含有转化型H-ras-1基因。其中1个基因的分子特征显示，第12位密码子是GAA而不是正常等位基因的GGA，编码谷氨酸而不是甘氨酸。因此，化学致癌作用是一个适当的模型来研究ras基因在人类肿瘤中的作用。
Each of 9 mammary carcinomas induced by a single injection of nitrosomethylurea into 50 day old Buf/N female rats, contained a transforming H-ras-1 gene. Molecular characterization of 1 of the genes revealed that the 12th codon was GAA instead of GGA of the normal allele, encoding glutamic acid in place of glycine. Thus, chemical carcinogenesis represents an adequate model to study the role of transforming ras genes in human neoplasia.