Induction of Mesenchymal Stem Cells-like Cells Derived from Human Gingival iPS Cells into Osteoblast-like Cells
Induction of Mesenchymal Stem Cells-like Cells Derived from Human Gingival iPS Cells into Osteoblast-like Cells
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人牙龈 iPS 细胞来源的间充质干细胞样细胞诱导成成骨细胞样细胞
DOI：
10.11223/jarde.15.85
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Naoyuki MATSUMOTO
中科院分区：
文献类型：
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作者：
Yuji NAKAYAMA;Yoshiya HASHIMOTO;Yoshitomo HONDA;Naoyuki MATSUMOTO
间充质干细胞（MSC）在体外增殖能力有限，因此很难获得足够的细胞数量用于植入。本研究的目的是诱导人牙龈诱导多能干细胞（HG-iPSCs）来源的MSC样细胞（MSLCs）向成骨样细胞分化。我们在无饲养层条件下从HG-iPSC衍生MSLC。当与骨形态发生蛋白（BMP）或无骨形态发生蛋白（BMP）培养时，用成骨细胞分化因子处理的表达MSC特异性标记物的MSLC表达成骨细胞特异性mRNA和蛋白水平。BMP-2对成骨分化早期指标的表达有积极作用。然而，不含BMP-2的培养基促进钙沉积，这发生在成骨分化的终末阶段。MSLCs可能具有更高的增殖能力，因此，体外分化的成骨样细胞和合适的支架可用于骨组织工程。
Mesenchymal stem cells (MSCs) have limited capacity to proliferate in vitro, making it very difficult to acquire sufficient cell numbers for implantation. The objective of this study was to induce MSC-like cells (MSLCs) derived from human gingival induced pluripotent stem (HG-iPSCs) into osteoblast-like cells. We derived MSLCs from HG-iPSCs under feeder-free conditions. MSLCs expressing MSC-specific markers treated with osteoblast differentiation factor expressed osteoblast-specific mRNA and protein level when cultured with or without bone morphogenetic protein (BMP). BMP-2 had a positive effect on the expression of early indicators of osteogenic differentiation. However, the medium without BMP-2 promoted calcium deposition, which occurs at the terminal stage of osteogenic differentiation. MSLCs are likely to have higher proliferative capacity, and hence, in vitro-differentiated osteoblast-like cells and appropriate scaffolds can be used for bone tissue engineering.