Morphological, histochemical, and immunocytochemical study of CO2 and Er:YAG laser effect on oral soft tissues.
Morphological, histochemical, and immunocytochemical study of CO2 and Er:YAG laser effect on oral soft tissues.
复制标题
CO2 和 Er:YAG 激光对口腔软组织影响的形态学、组织化学和免疫细胞化学研究。
DOI：
10.1089/1549541041438678
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发表时间：
2004
影响因子：
--
通讯作者：
A. Botticelli
中科院分区：
文献类型：
--
作者：
D. Zaffe;M. Vitale;A. Martignone;F. Scarpelli;A. Botticelli
目的
本研究的目的是探讨CO2或Er：YAG激光照射后口腔粘膜的形态学、组织化学和免疫细胞化学变化。
背景数据
目前还没有CO（2）和Er：YAG激光对人体口腔软组织作用的比较报道。
材料和方法
本文对40例青年患者经CO_2和Er：YAG激光手术获得的口腔活检组织进行了组织保存研究。在福尔马林固定、石蜡包埋活检组织的激光切割边缘进行苏木精-伊红和姬姆萨染色、PAS/琼脂糖酶处理、AE 1和AE 2细胞角蛋白、MiB 1/Ki 67和bcl-2免疫反应。
结果
CO2激光活检显示上皮有水疱、裂隙和糜烂。细胞内水肿，核延长。CO（2）激光活检的糖原含量结果降低。细胞角蛋白和细胞周期增殖标记在Er：YAG激光活检组织中表达较好，而细胞凋亡标记在CO（2）激光活检组织中表达较好。
结论
结果表明，Er：YAG激光对上皮组织损伤小，炎症反应轻，愈合快，瘢痕形成率低，可作为外科手术的常规方法。
OBJECTIVE
The purpose of this study was to investigate the morphological, histochemical, and immunocytochemical changes of the oral mucosa after CO(2) or Er:YAG laser irradiation.
BACKGROUND DATA
There have been no comparative reports on CO(2) and Er:YAG laser effects on human oral soft tissues.
MATERIALS AND METHODS
Tissue preservation was studied in 40 oral biopsies of young patients obtained by CO(2) and Er:YAG laser surgery. Hematoxylin-eosin and Giemsa stains, PAS/diastase treatment, AE1 and AE2 cytokeratins, MiB1/Ki67, and bcl-2 immunoreactions were performed on the laser cut edges on formalin fixed, paraffin embedded biopsies.
RESULTS
CO(2) laser biopsies show blisters, clefts and erosions of the epithelium. Intracellular edema and lengthened nuclei were also seen. The glycogen content results decreased in CO(2) laser biopsies. Good expression for cytokeratins and cell-cycle proliferation markers were found in Er:YAG biopsies, on the contrary the apoptosis marker was better expressed in CO(2) laser biopsies.
CONCLUSION
The results suggest that Er:YAG laser may be routinely used in surgery, because of its minimal damage of the epithelial tissue, its low inflammatory reaction, its quicker healing process and its lower risk of scarring.