Identification of upregulated genes in oral squamous cell carcinomas
Identification of upregulated genes in oral squamous cell carcinomas
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DOI：
10.1002/hed.23169
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发表时间：
2013-10-01
影响因子：
2.9
通讯作者：
Vettore, Andre Luiz
中科院分区：
文献类型：
--
作者：
Lessa, Roberta C.;Campos, Antonio Hugo J. F. M.;Vettore, Andre Luiz
口腔癌是头颈部鳞状细胞癌（HNSCC）中最常见的一种.这些肿瘤通常具有侵袭性的临床结果，其特征在于局部侵袭和区域淋巴结转移的倾向。上调的基因可作为诊断、预后的标志物，以及作为这些肿瘤的新的药物靶点。我们检查了ORESTES公共数据库，并使用定量逆转录聚合酶链反应（qRT-PCR）结果与结论ORESTES数据挖掘分析表明，40个基因在肿瘤组织中的表达水平与肿瘤组织中的表达水平呈显著正相关（P < 0.05在HNSCC中上调基因。这些候选基因中的9个被选择用于进一步的qRT-PCR验证，其中3个（ALDOA、AHSA 1和POLQ）在OSCC样品中经常被发现上调，这可能表明这些基因与该肿瘤部位的致癌过程相关，并且它们可以构成潜在的治疗新靶点。(c)2012 Wiley Periodicals，Inc.头颈部35：1475 - 1481，2013
BackgroundOral cancer is the most common subset of head and neck squamous cell carcinomas (HNSCC). These tumors often have an aggressive clinical outcome hallmarked by a propensity for local invasion and regional nodal metastasis. Upregulated genes could be useful as markers for diagnosis, prognosis, and as new drug targets for these tumors.MethodsTo identify upregulated genes in oral squamous cell carcinomas (OSSCs), we examined the ORESTES public database and used a quantitative reverse transcription-polymerase chain reaction (qRT-PCR) approach to determine the expression level of selected genes in tumor samples.Results and ConclusionsThe ORESTES data mining analysis indicated 40 upregulated genes in HNSCC. Nine of these candidate genes were selected for further qRT-PCR validation and 3 of them (ALDOA, AHSA1, and POLQ) were frequently found upregulated in OSCC samples, which may indicate an association of these genes with the carcinogenesis process in this tumor site and they can constitute potential new targets for therapy. (c) 2012 Wiley Periodicals, Inc. Head Neck 35: 1475-1481, 2013