Covalent structure of mutacin 1140 and a novel method for the rapid identification of lantibiotics
Covalent structure of mutacin 1140 and a novel method for the rapid identification of lantibiotics
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DOI：
10.1046/j.1432-1033.2000.01777.x
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发表时间：
2000-12-01
期刊：
影响因子：
--
通讯作者：
Edison, AS
中科院分区：
文献类型：
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作者：
Smith, L;Novák, J;Edison, AS
用核磁共振谱、质谱法和化学测序法确定了变形链球菌抗生素突变蛋白1140的一级结构。该结构与其他密切相关的抗生素一致，如表皮素。建立了一种新的方法，即用硼氢化钠和乙硫醇化学修饰突变蛋白1140，从而将含有硫醚的残留物与脱水残留物区分开来。这种双重标记策略提供了一种简单的方法，可以用最少的材料可靠地识别所有修饰的l抗生素残留物。虽然仍然需要核磁共振光谱学来获得硫醚桥联模式，从而获得完整的共价结构，但双标记技术与质谱学一起，在完整的核磁共振分析所需的一小部分时间内提供了大部分信息。因此，有了这些新技术，抗生素就可以快速地进行表征。
The primary structure of the Streptococcus mutans lantibiotic mutacin 1140 was elucidated by NMR spectroscopy, mass spectrometry, and chemical sequencing. The structure is in agreement with other closely related lantibiotics, such as epidermin. A novel method was developed in which mutacin 1140 was chemically modified with sodium borohydride followed by ethanethiol, allowing the differentiation of the thioether-containing residues from the dehydrated residues. This double-labeling strategy provides a simple method to reliably identify all modified lantibiotic residues with a minimal amount of material. While NMR spectroscopy is still required to obtain thioether bridging patterns and thus the complete covalent structure, the double-labeling technique, along with mass spectrometry, provides most of the information in a fraction of the time required for a complete NMR analysis. Thus, with these new techniques lantibiotics can be rapidly characterized.