Growth and spontaneous regression of swine melanoma: relationship of in vitro leukocyte reactivity.
Growth and spontaneous regression of swine melanoma: relationship of in vitro leukocyte reactivity.
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猪黑色素瘤的生长和自发消退：体外白细胞反应性的关系。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
R. Oxenhandler
中科院分区：
文献类型：
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作者：
J. Berkelhammer;B. Ensign;R. Hook;C. Hecker;G. D. Smith;R. Oxenhandler
应用视觉微细胞毒性技术评价黑素瘤猪对异基因猪黑素瘤靶细胞的白细胞反应性。在体内肿瘤生长和消退过程中不同时间采集的外周血白细胞在液氮中冷冻保存，当天解冻检测。纵向白细胞反应性与体内肿瘤体积的比较表明，黑色素瘤消退的猪在肿瘤消退过程中白细胞反应性增加。最大肿瘤体积小于30,000 mm3的猪表现出与体内肿瘤生长和消退模式相似的白细胞反应模式。然而，猪的肿瘤生长和消退最大。然而，最大肿瘤体积大于30,000 mm3的猪在体内肿瘤体积最大时，体外白细胞反应性增加。组织病理学分析显示，肿瘤体积的增加通常是宿主炎症细胞，特别是富含色素的巨噬细胞浸润肿瘤的结果。因此在肿瘤体积最大时，恶性黑素细胞数量呈比例减少，而含色素巨噬细胞呈比例增加。这些研究提供了额外的证据，证明猪黑色素瘤的自发肿瘤细胞消退与免疫事件有关，白细胞反应性测定在体外与宿主抗肿瘤免疫相关的研究中是有用的。
A visual microcytotoxicity technique was used to evaluate the leukocyte reactivity of melanoma swine against allogeneic swine melanoma target cells. Peripheral blood leukocytes, which had been collected and cryopreserved in liquid nitrogen at various times during in vivo tumor growth and regression, were thawed and tested on the same day. Comparison of longitudinal leukocyte reactivity with in vivo tumor volume indicated that swine with regressing melanomas exhibited increased leukocyte reactivity during tumor regression. Swine with maximum tumor volumes less than 30,000 mm3 exhibited patterns of leukocyte reactivity that paralleled the patterns of in vivo tumor growth and regression. However, swine with maximum tumor growth and regression. However, swine with maximum tumor volumes greater than 30,000 mm3 demonstrated increased in vitro leukocyte reactivity at the time of maximum in vivo tumor volume. Histopathologic analyses revealed that increases in tumor volume were frequently a result of host inflammatory cells, particularly pigment-laden macrophages, infiltrating the tumors. Thus at the time of maximum tumor volume, malignant melanocytes were proportionately decreasing in number while pigment-laden macrophages were proportionately increasing. These studies provide additional evidence that the spontaneous tumor cell regression of swine melanoma is associated with immunologic events and that assays of leukocyte reactivity are useful in vitro correlates of host antitumor immunity.