Highly Efficient FRET System Capable of Deep Photodynamic Therapy Established on X-ray Excited Mesoporous LaF3:Tb Scintillating Nanoparticles
Highly Efficient FRET System Capable of Deep Photodynamic Therapy Established on X-ray Excited Mesoporous LaF3:Tb Scintillating Nanoparticles
复制标题
X射线激发介孔LaF3:Tb闪烁纳米粒子建立高效FRET系统进行深度光动力治疗
DOI：
10.1021/acsami.5b03067
复制
发表时间：
2015-06-10
影响因子：
9.5
通讯作者：
Yang, Xiangliang
中科院分区：
文献类型：
--
作者：
Tang, Yong'an;Hu, Jun;Yang, Xiangliang
由于激发光在组织中的穿透深度有限，光动力学疗法（PDT）在很大程度上阻碍了对深部肿瘤的治疗。X射线被认为是一种理想的能量来源，可以在纳米颗粒（ScNP）的帮助下激活位于体内深处的光敏剂（PS）。然而，由于ScNP到PS的低发光和弱能量转移，在此概念下的功效不令人满意。在这里，介孔LaF 3：Tb ScNPs成功地合成了一个简单的水热过程中作为PS载体和X射线能量转换器，由于其良好的电离辐射阻止能力和高发光效率。用经典结晶理论详细阐述了多孔结构的形成机理。经过系统的研究，获得了具有优化闪烁发光的LaF 3：Tb ScNPs，用于负载孟加拉玫瑰红（RB），以建立有效的FRET系统，由于其良好的光谱匹配。SeNP和RB之间的FRET效率被计算为高达85%。在辐照下，通过FRET过程检测到LaF 3：Tb-RB纳米复合材料诱导的O-1（2）生成增强。该荧光共振能量转移系统在X射线光动力学治疗深部肿瘤中具有广阔的应用前景。
Photodynamic therapy (PDT) for deep-seated tumor is largely impeded by the limited penetration depth of excitation light in tissue. X-ray is considered as an ideal energy source to activate photosensitizers (PSs) located deep within the body with the assistance of scintillating nanoparticles (ScNPs). However, the efficacy under this concept is not satisfying due to the low scintillating luminescence and weak energy transfer from ScNPs to PSs. Here, mesoporous LaF3:Tb ScNPs were successfully synthesized by a facile hydrothermal process to act as PS carriers and X-ray energy transducers, owing to their good ionizing radiation stopping power and high luminescence efficiency. The formation mechanism of porous structure was elucidated in detail with classical crystallization theory. After a systematic investigation, LaF3:Tb ScNPs with optimized Scintillating luminescence were obtained for loading Rose Bengal (RB) to establish an efficient FRET system, owing to their excellent Spectral match. The FRET efficiency between SeNPs and RB was calculated to be as high as 85%. Under irradiation, enhanced O-1(2) generation induced by LaF3:Tb-RB nanocomposites via FRET process was detected. This LaF3:Tb-RB FRET system shows great potential to be applied in X-ray stimulated PDT for deep-seated tumors in the future.