Expression Patterns and Potential Biological Roles of Dip2a.
Expression Patterns and Potential Biological Roles of Dip2a.
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Dip2a 的表达模式和潜在生物学作用
DOI：
10.1371/journal.pone.0143284
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zheng Y
中科院分区：
文献类型：
--
作者：
Zhang L;Mabwi HA;Palange NJ;Jia R;Ma J;Bah FB;Sah RK;Li D;Wang D;Bah FB;Togo J;Jin H;Ban L;Feng X;Zheng Y
Disconnected（disco）-interacting protein 2 homolog A是Dip 2a基因编码的DIP 2蛋白家族的成员。Dip 2a的表达模式从未被系统地研究过。Dip 2a在胚胎发育和成体中的功能尚不清楚。为了研究Dip 2a基因在胚胎和成年中的表达和功能，通过使用CRISPR/Cas9技术在Dip 2a启动子后插入β-Gal cDNA来产生Dip 2a-LacZ小鼠模型。Dip 2a-LacZ小鼠被设计为lacZ报告小鼠以及Dip 2a敲除小鼠。杂合子小鼠用于研究内源性Dip 2a表达，纯合子用于研究DIP 2A相关的结构和功能。LacZ染色结果表明，Dip 2a广泛表达于神经、生殖和血管组织，以及心、肾、肝和肺。结果表明，Dip 2a在发育胚胎的外胚层来源的组织中表达。成人组织中的神经元、间充质细胞、内皮细胞、平滑肌细胞和心肌细胞按细胞类型显示丰富的染色。结果表明，DIP 2A的表达模式与FSTL 1的表达模式高度重叠，支持了DIP 2A可能是FSTL 1的受体并具有心肌保护作用的报道。Dip 2a在胚胎和成体中广泛而强烈的表达暗示了其多种结构和生理作用。
Disconnected (disco)-interacting protein 2 homolog A is a member of the DIP2 protein family encoded by Dip2a gene. Dip2a expression pattern has never been systematically studied. Functions of Dip2a in embryonic development and adult are not known. To investigate Dip2a gene expression and function in embryo and adult, a Dip2a-LacZ mouse model was generated by insertion of β-Gal cDNA after Dip2a promoter using CRISPR/Cas9 technology. Dip2a-LacZ mouse was designed to be a lacZ reporter mouse as well as a Dip2a knockout mouse. Heterozygous mice were used to study endogenous Dip2a expression and homozygotes to study DIP2A-associated structure and function. LacZ staining indicated that Dip2a is broadly expressed in neuronal, reproductive and vascular tissues, as well as in heart, kidney, liver and lung. Results demonstrate that Dip2a is expressed in ectoderm-derived tissues in developing embryos. Adult tissues showed rich staining in neurons, mesenchymal, endothelial, smooth muscle cells and cardiomyocytes by cell types. The expression pattern highly overlaps with FSTL1 and supports previous report that DIP2A to be potential receptor of FSTL1 and its protective roles of cardiomyocytes. Broad and intense embryonic and adult expression of Dip2a has implied their multiple structural and physiological roles.