Sensitization of T lymphocytes to thyroglobulin and thyroperoxidase in autoimmune thyroid diseases.
Sensitization of T lymphocytes to thyroglobulin and thyroperoxidase in autoimmune thyroid diseases.
复制标题
自身免疫性甲状腺疾病中 T 淋巴细胞对甲状腺球蛋白和甲状腺过氧化物酶的敏感性。
DOI：
10.3109/08916939309079227
复制
发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
R. Volpe
中科院分区：
文献类型：
--
作者：
F. Akasu;T. Morita;E. Resetkova;N. Yoshikawa;P. Carayon;R. Volpe
为了研究自身免疫性甲状腺疾病 (AITD) 中 T 细胞对甲状腺自身抗原的敏感性，使用了纯化的可溶性人甲状腺球蛋白 (Tg) 和甲状腺过氧化物酶 (TPO)。将来自 9 名格雷夫斯病 (GD) 患者、13 名桥本甲状腺炎 (HT) 和 10 名健康受试者的外周血单核细胞 (PBMC) 以及 CD8 耗尽、CD4 富集的 PBMC（“选定”PBMC）在有或没有不同浓度（分别为 0.1、1.0 和 5.0 微克/ml）的 Tg 或 TPO 的情况下培养 6 天，使用 3H-胸苷掺入测定评估他们的反应。总 PBMC 以及来自 GD 和 HT 的选定 PBMC 对 TPO 和 Tg 都有反应，但正常 PBMC 没有。这种诱导在“选定的”PBMC 中更为明显；另一方面，CD8 耗竭不允许正常 PBMC 对任一抗原作出反应。然而，选定的 AITD PBMC 对 Tg 的反应性与 TPO 不同。双向方差分析表明，在 GD 和 HT 中，Tg 的增殖反应显着大于 TPO（对于“选择的”PBMC 再次特别显着）。当使用不相关的（肾微粒体）抗原时，对照和 AITD 制剂之间没有差异。结合我们之前的报道，即使与 CD8 细胞一起培养，CD4 细胞也会被 TPO 诱导，很明显，抑制性 CD8 细胞确实在 CD4 细胞针对 Tg 和 TPO 的增殖中发挥了作用；然而，它们单独或与抑制-诱导CD4细胞组合的功能部分受到干扰，因此可以在AITD PBMC中鉴定T细胞对Tg和TPO的敏感性。
To investigate T-cell sensitization to thyroid autoantigens in autoimmune thyroid disease (AITD), purified soluble human thyroglobulin (Tg) and thyroid peroxidase (TPO) were used. Peripheral blood mononuclear cells (PBMC) as well as CD8-depleted, CD4-enriched PBMC ("selected" PBMC) from 9 patients with Graves' disease (GD), 13 Hashimoto's thyroiditis (HT) and 10 healthy subjects, were cultured for 6 days with or without varying concentrations (0.1, 1.0 and 5.0 micrograms/ml, respectively) of Tg or TPO and their responses were evaluated using the 3H-thymidine incorporation assay. Total PBMC as well as selected PBMC from GD and HT responded to both TPO and Tg, but normal PBMC did not. This induction was more marked in "selected" PBMC; on the other hand, CD8 depletion did not permit normal PBMC to respond to either antigen. However, reactivity of selected AITD PBMC to Tg differed from that of TPO. Two way analysis of variance showed that the proliferative response was significantly greater with Tg than with TPO, (again particularly notable with the "selected" PBMC) in both GD and HT. There was no difference between control and AITD preparations when an irrelevant (renal microsomal) antigen was employed. Taken together with our previous report that CD4 cells were induced by TPO even when cultured with CD8 cells, it is evident that suppressor CD8 cells do play a role in CD4 cells from proliferating against Tg and TPO; however their function alone or in combination with suppressor-inducer CD4 cells is partially disturbed, so that T cell sensitization to Tg and TPO can be identified in the AITD PBMC.
重症肌无力中 CD4 T 细胞对人乙酰胆碱受体 α 亚基的反应。
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Protti,MP;Manfredi,AA;Straub,C;HowardJr,JF;Conti-Tronconi,BM
通讯作者：
Conti-Tronconi,BM