Potent cross-group neutralization of primary human immunodeficiency virus isolates with monoclonal antibodies - Implications for acquired immunodeficiency syndrome vaccine
Potent cross-group neutralization of primary human immunodeficiency virus isolates with monoclonal antibodies - Implications for acquired immunodeficiency syndrome vaccine
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DOI：
10.1086/380102
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发表时间：
2004-01-01
影响因子：
6.4
通讯作者：
Ruprecht, RM
中科院分区：
文献类型：
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作者：
Ferrantelli, F;Kitabwalla, M;Ruprecht, RM
人类免疫缺陷病毒1型（HIV-1）在遗传学上分为组和进化枝（或亚型）。最初从HIV-1 M组进化枝B感染个体中分离的人中和单克隆抗体（nMAb）不仅在体外中和HIV-1进化枝B原代分离株，而且中和其他M组进化枝（A、C、D、E和F）的原代分离株。这纠正了以前认为的HIV-1主要分离株对中和抗体具有抗性的观点。在这里，我们表明，抗HIV-1 M组进化枝B nMAbs有效地中和了遗传学上远的HIV-1 O组的原代分离株。我们和其他人以前已经表明，被动免疫与人类nMAbs保护成年或新生灵长类动物感染的猴-人类免疫缺陷病毒株编码HIV-1组M-进化枝B包膜基因。本文所示的体外跨组中和强调了这些nMAb针对不同病毒变体的广泛潜力及其表位在获得性免疫缺陷综合征疫苗设计中的相关性。
Human immunodeficiency virus type 1 (HIV-1) is phylogenetically classified into groups and clades ( or subtypes). Human neutralizing monoclonal antibodies (nMAbs), originally isolated from individuals infected with HIV-1 group M-clade B, neutralized not only primary HIV-1 clade B isolates in vitro but also primary isolates of other group M clades ( A, C, D, E, and F). This corrected the previously held notion that primary HIV-1 isolates are resistant to neutralizing antibodies. Here we show that anti-HIV-1 group M-clade B nMAbs potently neutralized primary isolates of the phylogenetically distant HIV-1 group O. We and others have previously shown that passive immunization with human nMAbs protected adult or neonatal primates against infection with simian-human immunodeficiency virus strains encoding HIV-1 group M-clade B envelope genes. The in vitro cross-group neutralization shown here underscores the broad potential of these nMAbs against divergent virus variants and the relevance of their epitopes in the design of acquired immunodeficiency syndrome vaccines.