Vitamin E supplementation reverses the age-associated decrease in effective immune synapse formation in CD4+ T cells
Vitamin E supplementation reverses the age-associated decrease in effective immune synapse formation in CD4+ T cells
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DOI：
10.1196/annals.1331.059
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Meydani, SN
中科院分区：
文献类型：
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作者：
Ahmed, T;Marko, M;Meydani, SN
衰老与T细胞功能受损有关。我们在这里证明，与年龄相关的T细胞信号转导的下降是由于无法在T细胞受体和抗原相互作用的部位形成有效的免疫突触。基于我们之前对维生素E（VE）的研究，我们假设，老年CD4（+）T细胞的VE补充通过增加信号蛋白的易位来增强有效的免疫突触形成。使用共聚焦显微镜，我们发现当暴露于抗原递呈细胞时，老年小鼠的CD 4（+）T细胞与年轻小鼠的CD 4（+）T细胞相比，有效免疫突触的百分比较低。此外，我们发现，在体外和体内VE补充增加旧的CD4（+）T细胞能够形成一个功能性免疫突触的百分比。进一步的研究正在进行中，以确定年龄和VE诱导的有效免疫突触形成增强的机制。
Aging is associated with impairment of T cell function. We demonstrate here that age-associated declines in T cell signaling are due to the inability to form effective immune synapses at the site of the T cell receptor and antigen interaction. On the basis of our previous research with vitamin E (VE), we hypothesized that VE supplementation of old CD4(+) T cells enhances effective immune synapse formation through increased translocation of signaling proteins. Using confocal microscopy, we found that when exposed to antigenpresenting cells, CD4(+) T cells from old mice have a lower percentage of effective immune synapses compared to those from young mice. Furthermore, we show that in vitro and in vivo VE supplementation increases the percentage of old CD4(+) T cells capable of forming a functional immune synapse. Further studies are under way to determine the mechanisms of age and VE-induced enhancement of effective immune synapse formation.