MicroRNAs-424 and 206 are potential prognostic markers in spinal onset amyotrophic lateral sclerosis
MicroRNAs-424 and 206 are potential prognostic markers in spinal onset amyotrophic lateral sclerosis
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DOI：
10.1016/j.jns.2016.06.046
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发表时间：
2016-09-15
影响因子：
4.4
通讯作者：
Franca, Marcondes C., Jr.
中科院分区：
文献类型：
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作者：
de Andrade, Helen M. T.;de Albuquerque, Milena;Franca, Marcondes C., Jr.
简介：骨骼肌microRNAs（miRNAs）是肌萎缩侧索硬化（ALS）的潜在生物标志物，值得进一步研究。目的：鉴定ALS患者骨骼肌和血浆中miRNAs的异常表达，并将其与疾病进展参数相关联。随后，我们评估了一组39名患者/39名对照中候选miRNA的血浆表达。我们采用广义估计方程来研究与临床data.Results的相关性：我们确定了11个在ALS患者肌肉中差异表达的miRNA;其中，miR-424，miR-214和miR-206在肌肉样品中通过qPCR验证。在血浆中，我们发现只有miR-424和miR 206过表达。miR-424和miR-206的基线表达与ALS的临床恶化相关。结论：miR-424和miR-206是ALS潜在的预后标志物。(C)© 2016 Elsevier B. V.版权所有。
Introduction: Skeletal muscle microRNAs (miRNAs) are potential candidate biomarkers for amyotrophic lateral sclerosis (ALS) that deserve further investigation.Objectives: To identify miRNAs abnormally expressed in the skeletal muscle and plasma of patients with ALS, and to correlate them with parameters of disease progression.Methods: Expression profile of miRNAs in muscle was evaluated using an array platform. Subsequently we assessed the plasmatic expression of candidate miRNAs in a set of 39 patients/39 controls. We employed generalized estimating equations to investigate correlations with clinical data.Results: We identified 11 miRNAs differentially expressed in the muscle of ALS patients; of these, miR424, miR-214 and miR-206 were validated by qPCR in muscle samples. In plasma, we found only miR-424 and miR 206 to be overexpressed. Baseline expression of miR-424 and 206 correlated with clinical deterioration over time.Conclusion: MiR-424 and miR-206 are potential prognostic markers for ALS. (C) 2016 Elsevier B.V. All rights reserved.