Carotenoid accumulation and expression of carotenoid biosynthesis genes in mango flesh during fruit development and ripening
Carotenoid accumulation and expression of carotenoid biosynthesis genes in mango flesh during fruit development and ripening
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果实发育和成熟过程中芒果果肉中类胡萝卜素的积累和类胡萝卜素生物合成基因的表达
DOI：
10.1016/j.scienta.2018.04.009
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发表时间：
2018-07-14
影响因子：
4.3
通讯作者：
Wang, Songbiao
中科院分区：
文献类型：
--
作者：
Ma, Xiaowei;Zheng, Bin;Wang, Songbiao
为探讨芒果果肉类胡萝卜素生物合成的调控机制，以“台农1号”和“红玉”两个芒果品种为试材，分析了果实发育和成熟过程中果肉类胡萝卜素含量和15个类胡萝卜素基因的表达规律。两品种果实生长过程中类胡萝卜素积累量增加，采后成熟过程中类胡萝卜素含量急剧增加。成熟果实中，“台农1”果肉中类胡萝卜素总含量为177.16 μ g/g（鲜重）FW，“红玉”果肉中类胡萝卜素总含量为36.24 μ g/g FW。果肉中主要的类胡萝卜素是β-胡萝卜素和α-胡萝卜素，次要的类胡萝卜素是β-隐黄质、玉米黄质、叶黄素和新黄质。RT-PCR分析结果表明，两个品种果实中的CRTISO、PSY、ZDS、BCH和ZEP基因在果肉中表达上调，而LCYB、LCYE和NCED基因在果实发育和成熟过程中表达下调。CRTISO、PSY、ZDS、BCH和ZEP基因表达量与类胡萝卜素总量呈显著正相关，但品种间CRTISO基因表达量差异不显著。这些结果表明，PSY、ZDS、BCH和ZEP协同促进芒果果实发育成熟过程中类胡萝卜素的积累。
In order to investigate the regulation mechanisms of carotenoid biosynthesis in mango flesh, carotenoid content and the expression patterns of 15 carotenogenic gene in flesh of two mango cultivars 'Tainong1'and 'Hongyu' were analsyed during fruit development and ripening. Carotenoid were increasingly accumulated during fruit growth and sharply incaresed during postharvest ripening in both cultivars. In ripe fruit, total carotenoid content in the flesh of 'Tainongl' was 177.16 ug/g (Fresh weight) FW, and for 'Hongyu' 36.24 ug/gFW. The major carotenoids in the flesh were beta-Carotene and alpha carotene,with minor carotenoids being beta-cryptoxanthin, zeaxanthin, lutin and neoxanthin. RT-PCR analysis revealed that the expression of carotenogenic genes CRTISO, PSY, ZDS, BCH and ZEP were up-regulated in flesh, whereas the transcript levels of LCYB, LCYE and NCED were down-regulated in two cultivars and during fruit development and ripening. CRTISO, PSY, ZDS, BCH and ZEP transcripts were significantly positively correlated with the total carotenoids content, while no differences were detected for CRTISO expression between cultivars. These results suggested that PSY, ZDS, BCH and ZEP coordinately contribute to carotenoid accumulation during mango fruit development and ripening.