Activation of melanogenesis by vacuolar type H+-ATPase inhibitors in amelanotic, tyrosinase positive human and mouse melanoma cells
Activation of melanogenesis by vacuolar type H+-ATPase inhibitors in amelanotic, tyrosinase positive human and mouse melanoma cells
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DOI：
10.1016/s0014-5793(00)01795-6
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发表时间：
2000-07-28
期刊：
影响因子：
3.5
通讯作者：
Thody, AJ
中科院分区：
文献类型：
--
作者：
Ancans, J;Thody, AJ
在这项研究中，我们描述了选择性空泡型H+- atp酶抑制剂（巴菲霉素A1和康那霉素A）在表达酪氨酸酶但不显示黑素生成的无色性人和小鼠黑色素瘤细胞中激活黑素生成。添加抑制剂可在4小时内激活酪氨酸酶，24小时后细胞中含有可测量量的黑色素。环己亚胺（2 μ g/ml）不抑制这些作用，这与翻译后激活机制一致。我们的研究结果表明，黑素体pH值可能是控制哺乳动物细胞黑色素形成的一个重要的动态因素。(C) 2000年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
In this study, we describe the activation of melanogenesis by selective vacuolar type H+-ATPase inhibitors (bafilomycin A1 and concanamycin A) in amelanotic human and mouse melanoma cells which express tyrosinase but show no melanogenesis. Addition of the inhibitors activated tyrosinase within 4 h, and by 24 h the cells contained measurable amounts of melanin. These effects were not inhibited by cycloheximide (2 mu g/ml) which is consistent with a post-translational mechanism of activation. Our findings suggest that melanosomal pH could be an important and dynamic factor in the control of melanogenesis in mammalian cells. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.