Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage
Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage
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抗氧化酶和线粒体替代氧化酶途径在雨生红球藻绿色运动阶段的耐冷性中发挥重要作用
DOI：
10.1016/j.algal.2020.102003
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Chen Fangjian
中科院分区：
文献类型：
--
作者：
Zhang Chunhui;Li Ruizhi;Zhu Qin;Hang Wei;Zhang Hongjiang;Cui Hongli;Ji Chunli;Zhang Litao;Chen Fangjian
During outdoor large-scale cultivation,Haematococcus pluvialiscells, especially at the green motile stage, are frequently exposed to chilling stress in winter. However, the physiological responses and adaptation mechanisms to chilling inH. pluvialishave not been characterized. In order to better understand the chilling tolerance mechanisms ofH. pluvialisin the green motile stage, the responses of photosynthetic characteristics and photoprotective mechanisms to chilling were investigated. Chilling stress significantly decreased the activities of key enzymes (ribulose-1,5-bisphosphate carboxylase/oxygenase and NADP glyceraldehyde-3-phosphate dehydrogenase) in photosynthetic carbon assimilation, which would cause the accumulation of excess reducing equivalents and an imbalance of light absorption and energy utilization, leading to more severe photoinhibition. After chilling treatment, there was no induction of cyclic electron flow, non-photochemical quenching, fluorescence emission, or catalase, indicating these factors do not protectH. pluvialisfrom photoinhibition. However, chilling significantly enhanced the activities of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase), which efficiently dissipated excess electrons generated by photosynthetic linear electron flow (LEF). The malate-oxaloacetate shuttle was activated and the mitochondrial alternative oxidase (AOX) pathway was significantly up-regulated after chilling, indicating the transport of excess reducing equivalents generated by photosynthetic LEF to cytosol and mitochondria and oxidization by the AOX pathway, the mitochondrial non-phosphorylating pathway. As a result, the LEF was not inhibited by negative feedback and theH. pluvialiscells were well protected under chilling stress in the light, with no change in the effective quantum yield of PSII (ΦPSII) and the yield of non-regulated energy dissipation of PSII (ΦNO). The results indicate that antioxidant enzymes and the mitochondrial AOX pathway play important roles in protectingH. pluvialisat the green motile stage against chilling stress.
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