The domestication process of the Modern Rose: genetic structure and allelic composition of the rose complex
The domestication process of the Modern Rose: genetic structure and allelic composition of the rose complex
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DOI：
10.1007/s001220051660
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发表时间：
2001-02-01
影响因子：
5.4
通讯作者：
Heizmann, P
中科院分区：
文献类型：
--
作者：
Martin, M;Piola, F;Heizmann, P
采用随机引物PCR技术对13个园艺类群的100个老月季品种进行了遗传变异分析。使用5个长（20聚体）PCR引物，产生58个多态性DNA片段，其中55个是高度判别，允许100个品种的准整体的分化。构建了一个树状图，显示从PCR片段的存在/不存在估计的品种之间的相对遗传相似性。它展示了中国和欧洲创始人玫瑰之间的关系，第一代（Bourbons，Noiren，Portlands）和第二代（Hybrid Perpetuals和Teas）的杂交群体，以及驯化历史上生产的最现代的杂交茶。主成分分析（PCA）的相同的数据表明，欧洲/中国的等位基因比例的连续梯度的发生，和相当大的减少的遗传变异叠加驯化的进展。这两个互补的分析是在良好的协议与园艺文献。它们还提供了可能与控制不同群体主要形态发生特征的基因有关的DNA片段。
Genetic variability among 100 old cultivated rose varieties from 13 horticultural groups was estimated by arbitrary primed (AP) PCR. Using five long (20-mer) PCR primers, 58 polymorphic DNA fragments were produced, of which 55 were highly discriminant, allowing differentiation of the quasi-totality of the 100 cultivars. A dendrogram was constructed displaying the relative genetic similarities between cultivars estimated from the presence/absence of PCR fragments. It shows the relationships between the Chinese and European founder roses, hybrid groups of the first (Bourbons, Noisettes, Portlands) and second (Hybrid Perpetuals and Teas) generations, and the most modem Hybrid Teas, produced during the history of domestication. Principal components analysis (PCA) of the same data demonstrates the occurrence of a continuous gradient of the European/Chinese allele ratio, and a considerable reduction of genetic variability superimposed with the progress of domestication. The two complementary analyses are in good agreement with the horticultural literature. They also give access to DNA fragments potentially linked to genes involved in the control of the main morphogenetic characters of various groups.