Isolation, characterization and application of an alkaline resistant virulent bacteriophage JN01 against Escherichia coli O157:H7 in milk and beef
Isolation, characterization and application of an alkaline resistant virulent bacteriophage JN01 against Escherichia coli O157:H7 in milk and beef
复制标题
牛奶和牛肉中抗碱性强毒噬菌体 JN01 的分离、鉴定和应用
DOI：
10.1016/j.lwt.2021.111266
复制
发表时间：
2021-03-16
影响因子：
6
通讯作者：
Wu, Guo-Ping
中科院分区：
文献类型：
--
作者：
Li, Ya-Ke;Chen, Hu;Wu, Guo-Ping
噬菌体是防治食源性致病菌的一种新型生物防治工具。本研究以大肠杆菌（Escherichia coli，E. coli），命名为JN 01。该菌株对20株大肠杆菌中的16株具有裂解活性。大肠埃希菌中有11株为3种以上多重耐药菌株。生物学特性表明，JN 01的潜伏期为15 min，溶菌期为85 min。JN 01的爆发大小为每个感染细胞约134 PFU。JN 01在温度（30 ° C-70 ° C）和pH（4-13）等强制降解条件下相对稳定。JN 01的基因组为88，360-bp的双链DNA分子，共有130个开放阅读框（ORF），G + C含量为38.76%。重要的是，在JN 01的基因组中没有鉴定出已知的毒力相关、抗生素和溶原性相关基因。形态学和系统发育分析证实JN 01属于肌尾病毒科。E.活细胞计数大肠杆菌O 157：H7在牛奶和生牛肉样品中减少时，用JN 01在4 ℃处理。结果表明，JN 01是一种具有开发潜力的天然生防药剂。大肠杆菌O 157：H7。
Bacteriophage is a new biological control tool for the prevention and treatment of foodborne pathogens. In this study, an alkali-resistant phage against Escherichia coli (E. coli), named JN01, was isolated and characterized from wastewater. It exhibited lytic activities against 16 of 20 E. coli strains, 11 of which were more than three multidrug resistant strains. The biological characteristics of JN01 showed that the latent period and lysis period was 15 min and 85 min, respectively. The burst size of JN01 was about 134 PFU per infected cell. JN01 was relatively stable under stressed conditions such as temperatures (30 degrees C-70 degrees C) and pH (4-13). The genome of JN01 is 88,360-bp double-stranded DNA molecules, with a total of 130 open reading frames (ORFs) and 38.76% of G + C content. Importantly, no known virulence-associated, antibiotic and lysogeny-related genes were identified in the genome of JN01. Morphological and phylogenetic analyses confirmed that JN01 belongs to the Myoviridae. Viable cell counts of E. coli O157: H7 were reduced in milk and raw beef samples when treated with JN01 at 4 degrees C. The results indicated that JN01 could be a natural potential biological control agent for E. coli O157: H7 in foods.