Laser Raman studies of the 5 S rRNA‐protein L5 complex of rat liver ribosomes
Laser Raman studies of the 5 S rRNA‐protein L5 complex of rat liver ribosomes
复制标题
大鼠肝脏核糖体 5S rRNA-蛋白 L5 复合物的激光拉曼研究
DOI：
10.1016/0014-5793(82)80622-4
复制
发表时间：
1983
期刊：
影响因子：
3.5
通讯作者：
H. Welfle
中科院分区：
文献类型：
--
作者：
H. Fabian;S. Böhm;Wolfgang Carius;R. Misselwitz;H. Welfle
采用激光拉曼光谱技术研究了从大鼠肝脏核糖体中提取的5s rRNA - L5蛋白复合物中，核糖体蛋白L5对5s rRNA构象的影响。光谱比较显示，蛋白质诱导的5s rRNA的构象变化很小，但大多数碱基配对区域似乎存在于与蛋白质L5的复合物以及游离的5s rRNA中。此外，还指出了5s rRNA与L5蛋白之间的特异性相互作用。根据拉曼数据，单链区域的胞嘧啶（和/或尿嘧啶）残基和鸟嘌呤的N(7)参与与蛋白质的相互作用。
The effects of ribosomal protein L5 on the conformation of 5 S rRNA in the 5 S rRNA—protein L5 complex extracted from rat liver ribosomes have been studied by laser Raman spectroscopy. A comparison of the spectra shows small protein-induced conformational changes in the 5 S rRNA, but most of the base-paired regions appear to be present in the complex with protein L5 as well as in the free 5 S rRNA. Furthermore specific interactions between 5 S rRNA and protein L5 are indicated. Cytosine (and/or uracil) residues in single-stranded regions and the N(7) of guanine are engaged in interactions with the protein as suggested by the Raman data.
影响因子：
14.9
作者：
G. Studnicka;F. Eiserling;J. Lake
通讯作者：
G. Studnicka;F. Eiserling;J. Lake