Presence of exclusively bacteriochlorophyll-c containing substrain in the culture of green sulfur photosynthetic bacterium Chlorobium vibrioforme strain NCIB 8327 producing bacteriochlorophyll-d
Presence of exclusively bacteriochlorophyll-c containing substrain in the culture of green sulfur photosynthetic bacterium Chlorobium vibrioforme strain NCIB 8327 producing bacteriochlorophyll-d
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DOI：
10.2116/analsci.19.1575
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发表时间：
2003-12-01
影响因子：
1.6
通讯作者：
Tamiaki, H
中科院分区：
文献类型：
--
作者：
Saga, Y;Oh-Oka, H;Tamiaki, H
绿色硫光合细菌 Chlorobium (Chl.) vibrioforme f 的现有培养物中光捕获细菌叶绿素 (BChl) 的光依赖性组成变化。 sp。通过可见吸收光谱和 HPLC 分析研究了 thiosulfatophilum 菌株 NCIB 8327。当培养物在弱光条件下在液体培养基中重复生长时，体内光谱的 Soret 和 Q(y) 吸收带均移至更长的波长。通过HPLC对提取的色素进行分析表明，在反复培养过程中，BChl-c与BChl-d分子的量之比逐渐增加。相反，当在低光强度下生长的培养物转移到高光条件并继续生长时，吸收带移动到更短的波长并且BChls-c/d的比率最终下降到几乎原始值。在固体琼脂培养基上从含有 BChls-c 和 d 的液体培养物中制备菌落，该液体培养物在低光强度下生长。发现获得的每个菌落含有BChl-c或d，但不是两者都含有。根据基因分析判断，本研究中分离出的两种类型的细胞源自同一克隆。此处观察到的液体培养物中色素成分的变化可归因于分别含有 BChl-c 和 BChl-d 的两个亚菌株之间的生长速率差异，具体取决于光照条件。
The light-dependent composition change of light harvesting bacteriochlorophyll(BChl)s in the present culture of a green sulfur photosynthetic bacterium Chlorobium (Chl.) vibrioforme f. sp. thiosulfatophilum strain NCIB 8327 was investigated by visible absorption spectroscopy and HPLC analyses. When the culture was repeatedly grown in liquid media under a low light condition, both the Soret and Q(y) absorption bands of the in vivo spectrum were shifted to longer wavelengths. Analysis of the extracted pigments by HPLC revealed that the ratio of the amount of BChl-c to that of BChl-d molecules gradually increased during repeated cultivation. In contrast, when the culture grown under a low light intensity was transferred to a high light condition and continued to be grown, the absorption bands were shifted to shorter wavelengths and the ratio of BChls-c/d decreased finally to the almost original value. Colonies were prepared on solid agar media from the liquid culture containing both BChls-c and d, which was grown under a low light intensity. Each colony obtained was found to contain either BChl-c or d, but not both of them. Two types of cells isolated in this study were derived from the same clone, judged from their genetic analyses. The variation of pigment composition in our liquid culture observed here could be ascribed to the difference of growth rates between two substrains containing BChl-c and BChl-d, respectively, depending on light conditions.