Immunoassayable insulin content of subcellular fractions of rat islets.
Immunoassayable insulin content of subcellular fractions of rat islets.
复制标题
大鼠胰岛亚细胞部分的可免疫测定的胰岛素含量。
作者：
A. Lindall;M. W. Steffes;Robert L. Sorenson
通过改良的胶原酶消化程序从大鼠胰岛中分离胰岛。通过离心技术分离胰岛，并显示存在富集的胰岛素分泌颗粒级分以及胰岛素在其他级分中的定量分布。估计了各个级分中胰岛素相对于其他蛋白质的含量。针对这些结果，对目前有关胰岛和胰腺胰岛素含量的文献进行了讨论。（内分泌学85：218，1969）
Islets of Langerhans were isolated from rat islets by a modification of the collagenase digestion procedure. The islets were fractionated by centrifugation techniques and the presence of an enriched insulin secretion granule fraction was shown as well as the quantitative distribution of insulin in other fractions. The content of insulin relative to other proteins in individual fractions was estimated. Current literature on insulin content of islets and pancreas was discussed with respect to these results. (Endocrinology 85: 218, 1969)