A general procedure for the production of antibody reagents against eukaryotic ribosomal proteins
A general procedure for the production of antibody reagents against eukaryotic ribosomal proteins
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DOI：
10.2174/0929866054395860
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发表时间：
2005-08-01
影响因子：
1.6
通讯作者：
Ottonello, S
中科院分区：
文献类型：
--
作者：
Dieci, G;Bottarelli, L;Ottonello, S
尽管最近在细菌和古细菌核糖体的结构和功能分析方面取得了进展，但真核生物核糖体的结构和生物起源仍有待详细表征。核糖体蛋白特异性抗体将是此类研究的有价值的工具，但它们的产生通常受到真核核糖体蛋白在大肠杆菌中的表达和溶解性差的阻碍。杆菌我们在这里报告了一个改进的一般程序的过量生产重组真核核糖体蛋白和相应的多克隆抗体的产生。记录了通过该程序产生的抗体检测的特异性和灵敏度。
Despite recent progress in the structural and functional analysis of bacterial and archaeal ribosomes, the structure and biogenesis of eukaryotic ribosomes still awaits a detailed characterization. Ribosomal protein-specific antibodies would be valuable tools for such studies, but their production is commonly hindered by the poor expression and solubility of eukaryotic ribosomal proteins in E. coli. We report here an improved general procedure for the overproduction of recombinant eukaryotic ribosomal proteins and for the generation of the corresponding polyclonal antibodies. The specificity and sensitivity of detection of the antibodies produced by this procedure are documented.