A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding
A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding
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DOI：
10.1016/j.jcs.2009.05.006
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发表时间：
2009-09-01
影响因子：
3.8
通讯作者：
Ma, Wujun
中科院分区：
文献类型：
--
作者：
Liu, Li;Wang, Aili;Ma, Wujun
高分子量谷蛋白亚基在决定小麦面团质量中起着重要作用，因为它们赋予面团混合和烘焙性能所需的粘弹性。采用SDS-PAGE和MALDI-TOF-MS分析了来自12个国家的103个普通小麦基因型的HMW-GS的组成。结果表明，MALDI-TOF技术适用于大多数HMW-GS等位基因的分析。Glu-B1位点的等位基因多样性包括6+8b*、7、7+8、7+8a*、7b*+8、7（OE）、7(OE)+8、7(OE)+8a*、7(OE)+8b*、7+9、13+16、14+15、17+18和20亚基。讨论了MALDI-TOF-MS快速鉴定HMW-GS的能力及其在小麦选育计划中的筛选价值，特别是在鉴别与优质相关的7（OE）、8a*和8b*亚基方面的价值。在日本品种“真岛真木”中发现了一个新的谷蛋白亚基7b*+8。2009爱思唯尔有限公司版权所有。
High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7(OE), 7(OE)+8, 7(OE)+8a*, 7(OE)+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7(OE), 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki. (C) 2009 Elsevier Ltd. All rights reserved.