GENETIC-RELATIONSHIP BETWEEN 13 GENOME TYPES OF ADENOVIRUS 11, 34, AND 35 WITH DIFFERENT TROPISMS
GENETIC-RELATIONSHIP BETWEEN 13 GENOME TYPES OF ADENOVIRUS 11, 34, AND 35 WITH DIFFERENT TROPISMS
复制标题
DOI：
10.1159/000150218
复制
发表时间：
1991-11-01
期刊：
影响因子：
4.6
通讯作者：
WADELL, G
中科院分区：
文献类型：
--
作者：
LI, QG;HAMBRAEUS, J;WADELL, G
应用BamHI、Bcl I、BglII、BstEII、EcoRI、HindIII、HpaI、PstI、SalI、SmaI、XbaI和XhoI等13种限制性内切酶对20株从健康孕妇、尿路感染、呼吸道感染和咽结膜热患者分离的腺病毒血清型11（Ad11）进行了基因组分型。 所有的基因组类型可以根据它们的遗传同源性分为三个基因组簇，表示为成对共迁移的限制性片段。 基因组簇内的基因组类型非常密切相关。 它们平均共享91.6 - 97.7%的成对共迁移限制性片段。 基因组簇1和3的Ad11菌株从尿中分离，而从呼吸道分离的所有Ad11菌株被鉴定为基因组簇2的成员。 分别从出血性膀胱炎患者和器官移植受者中鉴定出一种基因组类型的Ad34和一种基因组类型的Ad35。 两者都与Ad11密切相关。 Ad35的基因组类型可能位于Ad11基因组簇1中。
Eleven genome types of adenovirus serotype 11 (Ad11) were identified among 20 strains isolated from healthy pregnant women and patients with urinary tract infections, respiratory tract infections, or pharyngoconjuctival fever by use of 13 restriction endonucleases: BamHI, BclI, BglII, BstEII, EcoRI, HindIII, HpaI, PstI, SalI, SmaI, XbaI, and XhoI. All genome types could be grouped into three genomic clusters according to their genetic homology expressed as pairwise comigrating restriction fragments. The genome types within a genomic cluster were very closely related. They shared on average pairwise comigrating restriction fragments of 91.6-97.7%. The Ad11 strains of genomic clusters 1 and 3 were isolated from urine, whereas all the Ad11 strains isolated from the respiratory tract were identified as members of the genomic cluster 2. One genome type of Ad34 and one genome type of Ad35 were identified from a hemorrhagic cystitis patient and an organ transplant recipient, respectively. Both were closely related to Ad11. The genome type of Ad35 could be located in the Ad11 genomic cluster 1.