rbohA a rice homologue of the mammalian gp91phox respiratory burst oxidase gene
rbohA a rice homologue of the mammalian gp91phox respiratory burst oxidase gene
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DOI：
10.1046/j.1365-313x.1996.10030515.x
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发表时间：
1996-09-01
期刊：
影响因子：
7.2
通讯作者：
Jones, JDG
中科院分区：
文献类型：
--
作者：
Groom, QJ;Torres, MA;Jones, JDG
据推测，植物含有呼吸爆发氧化酶，其在活化时氧化NADPH并产生细胞外超氧化物O-2（.-）这些蛋白质被认为在防御病原体方面发挥核心作用。然而，植物DNA序列编码的蛋白质与呼吸爆发氧化酶的组分相似，以前没有报道。本文报道了水稻rbohA（呼吸爆发氧化酶同源物）基因的全部cDNA和基因组DNA序列。预测的RbohA产品是最相似的主要催化亚基，gp 91 phox，呼吸爆发氧化酶的嗜中性粒细胞。逆转录酶PCR检测rbohA转录在根和芽的健康水稻植株。
It has been hypothesized that plants contain respiratory burst oxidases which, upon activation, oxidize NADPH and generate extracellular superoxide, O-2(.-) These proteins are proposed to play a central role in defence against pathogens. However, plant DNA sequences that encode proteins with similarity to components of respiratory burst oxidases have not previously been reported. This paper describes the complete cDNA and genomic DNA sequence of the rice rbohA (for respiratory burst oxidase homologue) gene. The predicted RbohA product is most similar to the main catalytic subunit, gp91phox, of the respiratory burst oxidase of neutrophils. Reverse transcriptase PCR detects rbohA transcripts in both roots and shoots of healthy rice plants.