Comparison of assurance gold salmonella EIA, BAX for screening/Salmonella, and GENE-TRAK Salmonella DLP rapid assays for detection of Salmonella in alfalfa sprouts and sprout irrigation water.
Comparison of assurance gold salmonella EIA, BAX for screening/Salmonella, and GENE-TRAK Salmonella DLP rapid assays for detection of Salmonella in alfalfa sprouts and sprout irrigation water.
复制标题
比较保证金沙门氏菌 EIA、用于筛查/沙门氏菌的 BAX 和 GENE-TRAK 沙门氏菌 DLP 快速检测法（用于检测苜蓿芽和芽灌溉水中的沙门氏菌）。
DOI：
10.1093/jaoac/85.2.395
复制
发表时间：
2002
影响因子：
1.6
通讯作者：
M. Tortorello
中科院分区：
文献类型：
--
作者：
D. Stewart;K. Reineke;M. Tortorello
对金沙门氏菌EIA、筛查/沙门氏菌BAX和基因-TRAK沙门氏菌DLP快速检测方法与《细菌分析手册》(BAM)中描述的用于分析是否存在沙门氏菌的紫花苜蓿芽和芽灌溉水的官方培养方法进行了比较。纯培养沙门氏菌的4个血清型(田纳西沙门氏菌、慕尼黑沙门氏菌、姆班丹卡沙门氏菌和古巴沙门氏菌)对Bax、gene-TRAK和Gold EIA的检测下限分别约为log102、5和6。尽管BAX的检测下限很低，但在分析受污染的芽苗和芽苗灌溉水方面，BAX的表现不如其他方法。对4个不同批次的芽苗和芽灌水样分别接种低[1~2个菌落单位]和高水平(68~180cfu/g)沙门氏菌，BAX法检出沙门氏菌58/(90.6%)，而基因-TRAK法、金标免疫法和BAM法检出/沙门氏菌(100%)。还比较了3批不同沙门氏菌暴发的紫花苜蓿发芽种子对自然污染的发芽和发芽灌溉水的分析性能。自然污染样品的阳性检测结果为金EIA 41，基因-TRAK 36，BAM 33和Bax 13。
The Assurance Gold Salmonella EIA, BAX for Screening/Salmonella, and GENE-TRAK Salmonella DLP rapid assays were compared with official cultural methods described in the Bacteriological Analytical Manual (BAM) for analysis of alfalfa sprouts and sprout irrigation water for the presence of Salmonella. The lower limits of detection of 4 serovars of Salmonella cells (S. tennessee, S. muenchen, S. mbandanka, and S. cubana) in pure culture were determined as approximately log10 2, 5, and 6 for the BAX, GENE-TRAK, and Gold EIA, respectively. Despite its low detection limit, the BAX did not perform as well as the other assays in analyzing contaminated sprouts and sprout irrigation water. For 4 different lots of sprouts and sprout irrigation water samples inoculated with the 4 serovars at low [1-2 colony forming units (CFU/g)] and high (68-180 CFU/g) levels, the BAX detected Salmonella in 58/64 (90.6%) of the samples, compared with 64/64 (100%) by the GENE-TRAK, Gold EIA, and BAM methods. Assay performance was also compared for analysis of naturally contaminated sprouts and sprout irrigation water with 3 lots of alfalfa sprouted seeds associated with different salmonellosis outbreaks. Positive assay results for the naturally contaminated samples were Gold EIA 41, GENE-TRAK 36, BAM 33, and BAX 13.