Study of the tumor microenvironment during breast cancer progression
Study of the tumor microenvironment during breast cancer progression
复制标题
DOI：
10.1186/s12935-017-0492-9
复制
发表时间：
2017-12-22
影响因子：
5.8
通讯作者：
Majidzadeh-A, Keivan
中科院分区：
文献类型：
--
作者：
Eftekhari, Rahil;Esmaeili, Rezvan;Majidzadeh-A, Keivan
背景：肿瘤微环境中的不同细胞和介质在乳腺癌的进展中发挥着重要作用。本研究的目的是确定肿瘤进展过程中微环境的组成，以发现新的相关生物标志物和靶向治疗的潜力。方法：在本研究中，收集了四个不同阶段的乳腺癌活检和对照乳腺活检。然后，测定与不同-CD4(+) T细胞亚群相关的几种标志物的mRNA表达，包括调节性T细胞(Treg)、辅助性T细胞(Th)1型、2型和17型。此外，我们还检测了两种炎症细胞因子（TNF-α和IL-6）以及FASL、IDO、SOCS1、VEGF和CCR7等炎症介质的表达。结果：结果显示，与对照组织相比，肿瘤组织中Th1和Th17基因的表达量降低。此外，我们发现与这两个细胞亚群相关的基因表达在癌症进展过程中下降。此外，TNF-a的表达水平随着肿瘤进展而增加。结论：我们得出结论，与免疫反应和炎症相关的基因的表达在肿瘤组织和对照组织之间是不同的。此外，这种差异在癌症的不同阶段持续存在。
Background: Different cells and mediators in the tumor microenvironment play important roles in the progression of breast cancer. The aim of this study was to determine the composition of the microenvironment during tumor progression in order to discover new related biomarkers and potentials for targeted therapy.Methods: In this study, breast cancer biopsies from four different stages, and control breast biopsies were collected. Then, the mRNA expression of several markers related to different -CD4(+) T cell subsets including regulatory T cells (Treg), T helper (Th) type 1, 2 and 17 were determined. In addition, we investigated the expression of two inflammatory cytokines (TNF-alpha and IL-6) and inflammatory mediators including FASL, IDO, SOCS1, VEGF, and CCR7.Results: The results showed that the expression of Th1 and Th17 genes was decreased in tumor tissues compared to control tissues. In addition, we found that the gene expression related to these two cell subsets decreased during cancer progression. Moreover, the expression level of TNF-a increased with tumor progression.Conclusion: We conclude that the expression of genes related to immune response and inflammation is different between tumor tissues and control tissues. In addition, this difference was perpetuated through the different stages of cancer.